Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development.
Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development.
复制标题
完全消毒后，链球菌突变体生物膜的残留结构有利于二级细菌粘附和生物膜重新开发。
DOI：
10.1371/journal.pone.0116647
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Okiji T
中科院分区：
文献类型：
--
作者：
Ohsumi T;Takenaka S;Wakamatsu R;Sakaue Y;Narisawa N;Senpuku H;Ohshima H;Terao Y;Okiji T
口腔生物膜的化学消毒通常使生物膜结构保持完整。本研究旨在检查残留结构是否促进继发性细菌粘附。在转盘反应器中的树脂复合盘上产生的变形链球菌生物膜用70%异丙醇完全消毒，并在重新供应相同的细菌溶液后在相同的反应器中再次培养。对标本进行荧光共聚焦激光扫描显微镜、活细胞计数和PCR-侵入测定，以观察和定量二次粘附的细胞。荧光显微镜分析，特别是纵向冷冻切片后，证实了消毒的生物膜结构上的活细胞的分层模式。试验标本的活细胞计数显著高于对照组，并随残留结构量和培养时间的增加而增加。线性回归分析显示活细胞计数和总细胞计数之间具有高度相关性。得出结论，消毒的生物膜结构有利于二次细菌粘附。
Chemical disinfection of oral biofilms often leaves biofilm structures intact. This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely with 70% isopropyl alcohol, and were again cultured in the same reactor after resupplying with the same bacterial solution. Specimens were subjected to fluorescence confocal laser scanning microscopy, viable cell counts and PCR-Invader assay in order to observe and quantify secondarily adhered cells. Fluorescence microscopic analysis, particularly after longitudinal cryosectioning, demonstrated stratified patterns of viable cells on the disinfected biofilm structure. Viable cell counts of test specimens were significantly higher than those of controls, and increased according to the amount of residual structure and culture period. Linear regression analysis exhibited a high correlation between viable and total cell counts. It was concluded that disinfected biofilm structures favored secondary bacterial adhesion.
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