Elastic Laminar Reorganization Occurs with Outward Diameter Expansion during Collateral Artery Growth and Requires Lysyl Oxidase for Stabilization.
Elastic Laminar Reorganization Occurs with Outward Diameter Expansion during Collateral Artery Growth and Requires Lysyl Oxidase for Stabilization.
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DOI：
10.3390/cells11010007
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发表时间：
2021-12-21
期刊：
影响因子：
6
通讯作者：
Tzeng E
中科院分区：
文献类型：
--
作者：
McEnaney RM;McCreary DD;Skirtich NO;Andraska EA;Sachdev U;Tzeng E
当大动脉闭塞时，血流动力学变化刺激动脉网络重塑，形成侧支动脉，这一过程称为动脉生成。然而，侧支重塑所需的结构变化尚未定义。我们假设，细胞外基质的解构对于将较小的动脉重塑为有效的侧支至关重要。用多光子显微镜对大鼠缺血后肢成熟侧支动脉的胶原和弹性蛋白结构进行了分析。在不同时间点采集的侧支动脉显示出进行性的内径扩张，伴随着内弹力板(IEL)的显著重排成疏松的纤维网，这种模式持续到8周。尽管管腔直径增加了2.5倍，侧支动脉中的总弹性蛋白含量与对照动脉相比保持不变。侧支中间带中，基线弹性纤维含量较低。这些直径增加10-20倍的血管向外重构与弹性纤维断裂和壁张力增加有关，外膜胶原的伸直证明了这一点。用β-氨基丙腈抑制赖氨酰氧化酶(LOX)的功能，导致发育侧支的IEL严重碎裂或完全丧失连续性。侧支动脉的发展与现有弹性纤维的永久性重新分布有关，以适应直径的增长。我们没有发现新的弹性纤维形成的证据。外向重塑过程中动脉壁的稳定是必要的，并且依赖于LOX的活性。
When a large artery becomes occluded, hemodynamic changes stimulate remodeling of arterial networks to form collateral arteries in a process termed arteriogenesis. However, the structural changes necessary for collateral remodeling have not been defined. We hypothesize that deconstruction of the extracellular matrix is essential to remodel smaller arteries into effective collaterals. Using multiphoton microscopy, we analyzed collagen and elastin structure in maturing collateral arteries isolated from ischemic rat hindlimbs. Collateral arteries harvested at different timepoints showed progressive diameter expansion associated with striking rearrangement of internal elastic lamina (IEL) into a loose fibrous mesh, a pattern persisting at 8 weeks. Despite a 2.5-fold increase in luminal diameter, total elastin content remained unchanged in collaterals compared with control arteries. Among the collateral midzones, baseline elastic fiber content was low. Outward remodeling of these vessels with a 10–20 fold diameter increase was associated with fractures of the elastic fibers and evidence of increased wall tension, as demonstrated by the straightening of the adventitial collagen. Inhibition of lysyl oxidase (LOX) function with β-aminopropionitrile resulted in severe fragmentation or complete loss of continuity of the IEL in developing collaterals. Collateral artery development is associated with permanent redistribution of existing elastic fibers to accommodate diameter growth. We found no evidence of new elastic fiber formation. Stabilization of the arterial wall during outward remodeling is necessary and dependent on LOX activity.
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DOI：
10.1152/ajpheart.00478.2002
发表时间：
2003-01-01
影响因子：
4.8
作者：
Cai, WJ;Koltai, S;Schaper, J
通讯作者：
Schaper, J
影响因子：
3.9
作者：
Maniaci A;Iannella G;Cocuzza S;Vicini C;Magliulo G;Ferlito S;Cammaroto G;Meccariello G;De Vito A;Nicolai A;Pace A;Artico M;Taurone S
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Taurone S
影响因子：
3.6
作者：
Kulkarni R;Andraska E;McEnaney R
通讯作者：
McEnaney R
影响因子：
4.6
作者：
Alves, Calebe;Araujo, Ascanio D.;Suki, Bela
通讯作者：
Suki, Bela
影响因子：
5
作者：
Dodd T;Jadhav R;Wiggins L;Stewart J;Smith E;Russell JC;Rocic P
通讯作者：
Rocic P