Gonadotropin-releasing hormone messenger ribonucleic acid and protein expression in Vero cells.
Gonadotropin-releasing hormone messenger ribonucleic acid and protein expression in Vero cells.
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Vero 细胞中促性腺激素释放激素信使核糖核酸和蛋白质的表达。
DOI：
10.1023/a:1016670402390
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发表时间：
2001
影响因子：
3.1
通讯作者：
Polan,ML
中科院分区：
文献类型：
--
作者：
Huang,HY;Raga,F;Kruessel,JS;Wen,Y;Soong,YK;Polan,ML
Purpose: Interleukin-1 (IL-1) is a major regulator of local cellular interactions during embryonic implantation. We hypothesized that gonadotropin-releasing hormone (GnRH) may also play a role in the embryonic/epithelial dialogue during early implantation. To examine this hypothesis, we examined the ability of IL-1 to regulate GnRH mRNA and protein expression in Vero cells.Methods: Viable Vero cells (1 × 105/well) were cultured in multiple-well tissue culture plates for in vitro studies and in 4-well chamber slides for immunohistochemical study. Confluent Vero cells were cultured with increasing concentrations of recombinant human IL-1β for an additional 24 hr. Vero cell expression of GnRH and GnRH receptor mRNAs was measured with polymerase chain reaction (PCR) and nested PCR, respectively. GnRH protein expression was validated by immunohistochemistry study. The quantitative level of GnRH mRNA expression regulated by IL-1β in Vero cells was determined by quantitative competitive PCR (QC PCR) with standard curve methodology.Results: RT-PCR revealed β-actin, GnRH, and GnRH receptor mRNA expression in Vero cell cultures. Immunostaining confirmed the presence of GnRH protein in Vero cells. Quantitative PCR demonstrated IL-1β up-regulation of Vero cell GnRH mRNA expression (p < 0.05).Conclusions: These results suggest that Vero cell mRNA and protein expression of GnRH may play a substantial role in early embryo/epithelial dialogue during embryo coculture, with an embryotrophic effect due to expression of GnRH by Vero cells.
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DOI：
10.1210/jcem.79.3.8077380
发表时间：
1994-09
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Elizabeth A. Stewart;Andrew J. Friedman;Kimberly Peck;Romana A. Nowak
通讯作者：
Elizabeth A. Stewart;Andrew J. Friedman;Kimberly Peck;Romana A. Nowak
人子宫肌层和平滑肌瘤中表皮生长因子和胰岛素样生长因子 I 的结合
影响因子：
7.2
作者：
PÄIVI TOMMOLA;F. Pekonen;E. Rutanen
通讯作者：
E. Rutanen
用于测量基因表达的定量 RT-PCR。
影响因子：
2.7
作者：
Mary C. Riedy;Timm Ea;Stewart Cc
通讯作者：
Stewart Cc
用于通过 RT-PCR 检测和定量生长因子、细胞因子、细胞外基质成分和基质金属蛋白酶的竞争性 RNA 模板。
影响因子：
2.7
作者：
Tarnuzzer,RW;Macauley,SP;Farmerie,WG;Caballero,S;Ghassemifar,MR;Anderson,JT;Robinson,CP;Grant,MB;Humphreys-Beher,MG;Franzen,L;Peck,AB;Schultz,GS
通讯作者：
Schultz,GS
CYP19基因及其产物芳香酶细胞色素P450在人子宫肌瘤组织和培养细胞中的表达。
DOI：
10.1210/jcem.78.3.8126151
发表时间：
1994
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Bulun,SE;Simpson,ER;Word,RA
通讯作者：
Word,RA