Optical imaging of fibrin deposition to elucidate participation of mast cells in foreign body responses.
Optical imaging of fibrin deposition to elucidate participation of mast cells in foreign body responses.
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DOI：
10.1016/j.biomaterials.2013.11.040
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发表时间：
2014-02
期刊：
影响因子：
14
通讯作者：
Tang, Liping
中科院分区：
文献类型：
--
作者：
Tsai, Yi-Ting;Zhou, Jun;Weng, Hong;Tang, Ewin N.;Baker, David W.;Tang, Liping
肥大细胞活化已被证明是引发和异物反应的关键决定因素。然而，目前还没有一种非侵入性的方法可以量化植入体相关肥大细胞的激活程度。利用纤维蛋白沉积是生物材料植入物周围肥大细胞活化的标志这一事实，制备了一种对纤维蛋白具有高亲和力的近红外探针。随后的体外测试证实该探针对纤维蛋白具有高亲和力。通过皮下颗粒植入模型，我们发现早在颗粒植入后15分钟，纤维蛋白亲和探针就在植入部位显著积累。如果在植入部位施用抗凝血肝素，纤维蛋白亲和探针在植入部位的积累也可以大大减少。进一步的研究表明，皮下注射肥大细胞激活剂-化合物48/80 -促进纤维蛋白亲和探针的积累。然而，在肥大细胞缺乏的小鼠中，植入物相关的纤维蛋白亲和探针积累显著减少。结果表明，我们的纤维蛋白亲和探针可以作为一种强有力的工具来监测和测量生物材料介导的纤维蛋白沉积和肥大细胞活化的程度。
Mast cell activation has been shown to be an initiator and a key determinant of foreign body reactions. However, there is no non-invasive method that can quantify the degree of implant-associated mast cell activation. Taking advantage of the fact that fibrin deposition is a hallmark of mast cell activation around biomaterial implants, a near infrared probe was fabricated to have high affinity to fibrin. Subsequent in vitro testing confirmed that this probe has high affinity to fibrin. Using a subcutaneous particle implantation model, we found significant accumulation of fibrin-affinity probes at the implant sites as early as 15 min following particle implantation. The accumulation of fibrin-affinity probes at the implantation sites could also be substantially reduced if anti-coagulant – heparin was administered at the implant sites. Further studies have shown that subcutaneous administration of mast cell activator – compound 48/80 – prompted the accumulation of fibrin-affinity probes. However, implant-associated fibrin-affinity probe accumulation was substantially reduced in mice with mast cell deficiency. The results show that our fibrin-affinity probes may serve as a powerful tool to monitor and measure the extent of biomaterial-mediated fibrin deposition and mast cell activation in vivo.
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DOI：
10.1073/pnas.88.3.1044
发表时间：
1991-02-01
影响因子：
11.1
作者：
LOIKE, JD;SODEIK, B;SILVERSTEIN, SC
通讯作者：
SILVERSTEIN, SC
影响因子：
3.4
作者：
Neeves, K. B.;Illing, D. A. R.;Diamond, S. L.
通讯作者：
Diamond, S. L.
DOI：
10.1161/atvbaha.111.225334
发表时间：
2011-06-01
影响因子：
8.7
作者：
Cooley, Brian C.
通讯作者：
Cooley, Brian C.
影响因子：
15
作者：
Pan, Dipanjan;Caruthers, Shelton D.;Lanza, Gregory M.
通讯作者：
Lanza, Gregory M.
影响因子：
4.7
作者：
McCarthy JR;Patel P;Botnaru I;Haghayeghi P;Weissleder R;Jaffer FA
通讯作者：
Jaffer FA