Comparison of transitional vs surgical menopause on monoamine and amino acid levels in the rat brain.
Comparison of transitional vs surgical menopause on monoamine and amino acid levels in the rat brain.
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DOI：
10.1016/j.mce.2018.05.003
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发表时间：
2018-11-15
影响因子：
4.1
通讯作者：
Gibbs RB
中科院分区：
文献类型：
--
作者：
Long T;Yao JK;Li J;Kirshner ZZ;Nelson D;Dougherty GG;Gibbs RB
卵巢功能丧失对神经递质的产生和释放有重要影响，并对认知能力产生相应的影响。迄今为止，几乎没有直接比较手术和过渡性绝经对大脑神经递质通路的影响。在这项研究中，评估了成年卵巢切除 (OVX) 大鼠和每日注射 4-乙烯基环己烯二环氧化物 (VCD) 选择性逐渐去除卵泡的大鼠对单胺、单胺代谢物以及氨基酸色氨酸 (TRP) 和酪氨酸 (TYR) 的影响。在 OVX 或 VCD 治疗后 1 周和 6 周时，对海马 (HPC)、额叶皮质 (FCX) 和纹状体 (STR) 的组织进行解剖和分析。在发情前期和发情间期收集性腺完整的大鼠的组织，以代表高雌激素和低雌激素自然状态下的神经化学水平。在性腺完整的大鼠中，FCX 发情前期检测到的血清素 (5-HT) 水平高于发情间期。此外，FCX 和 HPC 中 5-羟基吲哚乙酸 (5-HIAA)/5HT 的比率在发情前期低于发情间期，表明这些区域对 5-HT 周转有影响。没有观察到发情前期和发情间期之间的其他显着差异。在 OVX 和 VCD 治疗的大鼠中，观察到了与大脑区域和时间点相关的变化。在 OVX 和 VCD 治疗的大鼠中，去甲肾上腺素、5-HIAA、TRP 和 TYR 的 HPC 水平在 1 周时显着降低，但在 6 周时，OVX 和 VCD 处理的大鼠相对于发情前期和发情间期均没有显着降低。在 FCX 中，相对于动情间期，OVX 后 6 周多巴胺水平升高。 VCD 治疗后 1 周（但不是 6 周）观察到类似的趋势。在 STR 中，相对于发情前期和发情间期，OVX 后 1 周去甲肾上腺素水平升高，VCD 治疗后 1 周（但不是 6 周）HVA 水平升高。总的来说，这些数据首次提供了比较两种更年期模型对大脑多个神经内分泌终点的影响的全面分析。据报道，这些影响可能会导致手术和过渡性更年期对大脑功能和认知能力的影响。
Loss of ovarian function has important effects on neurotransmitter production and release with corresponding effects on cognitive performance. To date, there has been little direct comparison of the effects of surgical and transitional menopause on neurotransmitter pathways in the brain. In this study, effects on monoamines, monoamine metabolites, and the amino acids tryptophan (TRP) and tyrosine (TYR) were evaluated in adult ovariectomized (OVX) rats and in rats that underwent selective and gradual ovarian follicle depletion by daily injection of 4-vinylcyclohexene-diepoxide (VCD). Tissues from the hippocampus (HPC), frontal cortex (FCX), and striatum (STR) were dissected and analyzed at 1- and 6-weeks following OVX or VCD treatments. Tissues from gonadally intact rats were collected at proestrus and diestrus to represent neurochemical levels during natural states of high and low estrogens. In gonadally intact rats, higher levels of serotonin (5-HT) were detected at proestrus than at diestrus in the FCX. In addition, the ratio of 5-hydroxyindoleacetic acid (5-HIAA)/5HT in the FCX and HPC was lower at proestrus than at diestrus, suggesting an effect on 5-HT turnover in these regions. No other significant differences between proestrus and diestrus were observed. In OVX- and VCD-treated rats, changes were observed which were both brain region- and time point-dependent. In the HPC levels of norepinephrine, 5-HIAA, TRP and TYR were significantly reduced at 1 week, but not 6 weeks, in both OVX and VCD-treated rats relative to proestrus and diestrus. In the FCX, dopamine levels were elevated at 6 weeks after OVX relative to diestrus. A similar trend was observed at 1 week (but not 6 weeks) following VCD treatment. In the STR, norepinephrine levels were elevated at 1 week following OVX, and HVA levels were elevated at 1 week, but not 6 weeks, following VCD treatment, relative to proestrus and diestrus. Collectively, these data provide the first comprehensive analysis comparing the effects of two models of menopause on multiple neuroendocrine endpoints in the brain. These effects likely contribute to effects of surgical and transitional menopause on brain function and cognitive performance that have been reported.
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影响因子：
3.7
作者：
Ding F;Yao J;Zhao L;Mao Z;Chen S;Brinton RD
通讯作者：
Brinton RD
影响因子：
4.8
作者：
BEATTIE, CW;SOYKA, LF;RODGERS, CH
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RODGERS, CH
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作者：
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Bimonte-Nelson, Heather A.
影响因子：
3.7
作者：
BITAR, MS;OTA, M;SHAPIRO, BH
通讯作者：
SHAPIRO, BH
影响因子：
3.5
作者：
Heikkinen, T;Puoliväli, J;Tanila, H
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Tanila, H