Sequence identification, chromosomal mapping and tissue specific expression of the porcine paraoxonase 1 (PON1) gene
Sequence identification, chromosomal mapping and tissue specific expression of the porcine paraoxonase 1 (PON1) gene
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猪对氧磷酶 1 (PON1) 基因的序列鉴定、染色体作图和组织特异性表达
DOI：
10.1007/s11033-009-9514-6
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发表时间：
2010-03
期刊：
影响因子：
--
通讯作者：
李加琪
中科院分区：
文献类型：
--
作者：
李加琪
血清对氧磷酶（PON1）在保护低密度脂蛋白免受氧化修饰中起重要作用。本研究采用反转录聚合酶链反应和快速扩增cDNA末端的方法，克隆了猪PON 1的全长cDNA序列（1，416 bp）。发现它包含一个1，068 bp的开放阅读框，编码355个氨基酸的推导蛋白，计算分子量为40.02 kDa。利用细菌人工染色体克隆对中国二花脸猪PON1基因组结构和序列进行了分析。猪PON1基因由9个外显子和8个内含子组成，大小约29 kb，经IMpRH定位，位于第9号染色体微卫星标记SWR915和SW944之间。猪PON1在肾脏中表达量最高，其次是肝脏、肺和小肠，在心脏中表达量极低，在脾脏、淋巴、前肢肌肉、大脑、脂肪、小脑和下丘脑中几乎不表达。
Serum paraoxonase (PON1) plays an important role in protecting low-density lipoprotein against oxidative modification. In this study, the full-length cDNA sequence (1,416 bp) of porcinePON1was cloned by reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. It was found to contain a 1,068 bp open reading frame encoding a deduced protein of 355 amino acids with a calculated molecular weight of 40.02 kDa. The genomic structure and sequence of porcinePON1were also analyzed using a bacterial artificial chromosome clone of a Chinese Erhualian pig. The porcinePON1gene contained nine exons and eight introns spanning approximately 29 kb, and was located on chromosome nine between microsatellite markersSWR915andSW944by IMpRH mapping. PorcinePON1was highly expressed in kidney, followed by liver, lung and small intestine, expressed at an extremely low level in heart, and was hardly expressed in spleen, lymph, muscle of the anterior limb, cerebrum, fat, cerebellum or hypothalamus.
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影响因子：
5.5
作者：
B. Mackness;M. Mackness;P. Durrington;S. Arrol;A. Evans;D. Mcmaster;J. Ferrières;J. Ruidavets;Williams Nr;A. Howard
通讯作者：
B. Mackness;M. Mackness;P. Durrington;S. Arrol;A. Evans;D. Mcmaster;J. Ferrières;J. Ruidavets;Williams Nr;A. Howard
影响因子：
64.8
作者：
A. Lusis
通讯作者：
A. Lusis
DOI：
10.1016/s1567-5688(01)80161-6
发表时间：
2001-02
期刊：
The Biochemical journal
影响因子：
--
作者：
L. Rodrigo;B. Mackness;P. Durrington;Antonio F. Hernández;M. Mackness
通讯作者：
L. Rodrigo;B. Mackness;P. Durrington;Antonio F. Hernández;M. Mackness
影响因子：
2.9
作者：
KELSO, GJ;STUART, WD;HARMONY, JAK
通讯作者：
HARMONY, JAK
影响因子：
6.1
作者：
Tanimoto, N;Kumon, Y;Hashimoto, K
通讯作者：
Hashimoto, K