The Shape of the Jaw-Zebrafish Col11a1a Regulates Meckel's Cartilage Morphogenesis and Mineralization.
The Shape of the Jaw-Zebrafish Col11a1a Regulates Meckel's Cartilage Morphogenesis and Mineralization.
复制标题
颌骨Col11a1a的形状调节了梅克尔的软骨形态发生和矿化。
DOI：
10.3390/jdb10040040
复制
发表时间：
2022-09-22
影响因子：
2.7
通讯作者：
Oxford, Julia Thom
中科院分区：
文献类型：
--
作者：
Reeck, Jonathon C.;Oxford, Julia Thom
关键词：
col11a1a基因的表达对正常骨骼发育至关重要，影响软骨和骨骼。功能突变的丧失已被证明会导致长骨生长板以及颅面发育的异常。然而，对梅克尔软骨的具体影响尚未得到很好的研究。为了进一步了解col11a1a基因功能的影响，我们利用转基因Tg（sp7:EGFP）和Tg(Fli1a:EGFP) EGFP报告鱼以及野生型AB斑马鱼，通过注射反义morpholino寡核苷酸敲低该基因来分析斑马鱼发育中的下颌。我们的研究结果表明，斑马鱼col11a1a基因敲低会损害发育中的颌骨中梅克尔软骨的细胞组织，并改变梅克尔软骨附近的骨形成。这些结果表明，Col11a1a蛋白在骨骼发育的软骨中间体、长骨骨领随后的矿化以及发育中的下颌梅克尔软骨附近发生的矿化中发挥了作用。
The expression of the col11a1a gene is essential for normal skeletal development, affecting both cartilage and bone. Loss of function mutations have been shown to cause abnormalities in the growth plate of long bones, as well as in craniofacial development. However, the specific effects on Meckel’s cartilage have not been well studied. To further understand the effect of col11a1a gene function, we analyzed the developing jaw in zebrafish using gene knockdown by the injection of an antisense morpholino oligonucleotide using transgenic Tg(sp7:EGFP) and Tg(Fli1a:EGFP) EGFP reporter fish, as well as wildtype AB zebrafish. Our results demonstrate that zebrafish col11a1a knockdown impairs the cellular organization of Meckel’s cartilage in the developing jaw and alters the bone formation that occurs adjacent to the Meckel’s cartilage. These results suggest roles for Col11a1a protein in cartilage intermediates of bone development, the subsequent mineralization of the bony collar of long bones, and that which occurs adjacent to Meckel’s cartilage in the developing jaw.
登录
查看更多内容
影响因子：
1.5
作者：
DeLaurier, April;Eames, B. Frank;Blanco-Sanchez, Bernardo;Peng, Gang;He, Xinjun;Swartz, Mary E.;Ullmann, Bonnie;Westerfield, Monte;Kimmel, Charles B.
通讯作者：
Kimmel, Charles B.
影响因子：
2.7
作者：
Hafez A;Squires R;Pedracini A;Joshi A;Seegmiller RE;Oxford JT
通讯作者：
Oxford JT
影响因子：
2.7
作者：
Hardy MJ;Reeck JC;Fang M;Adams JS;Oxford JT
通讯作者：
Oxford JT
影响因子：
3.5
作者：
Akawi, N. A.;Al-Gazali, L.;Ali, B. R.
通讯作者：
Ali, B. R.
影响因子：
2
作者：
Hufnagel, Sophia B.;Weaver, K. Nicole;Hopkin, Robert J.
通讯作者：
Hopkin, Robert J.