Zebrafish sp7:EGFP: a transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration.
Zebrafish sp7:EGFP: a transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration.
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DOI：
10.1002/dvg.20639
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发表时间：
2010-08
期刊：
影响因子：
1.5
通讯作者：
Kimmel, Charles B.
中科院分区：
文献类型：
--
作者：
DeLaurier, April;Eames, B. Frank;Blanco-Sanchez, Bernardo;Peng, Gang;He, Xinjun;Swartz, Mary E.;Ullmann, Bonnie;Westerfield, Monte;Kimmel, Charles B.
我们报告的表达模式和建设的转基因斑马鱼线的转录因子参与耳泡的形成和骨骼。锌指转录因子sp7（以前称为osterix）被报道为成骨细胞的标志物。利用细菌人工染色体（BAC）介导的转基因技术，我们获得了一个用于研究骨骼发育的转基因斑马鱼品系Tg（sp7：EGFP）b1212。使用斑马鱼BAC，将EGFP引入sp7调控区的下游，并注射到1细胞期胚胎中。在该转基因系中，GFP表达再现了耳基板和囊泡中的内源性sp7基因表达，并形成骨骼结构。GFP阳性细胞也在成鱼中检测到，并发现与截肢后再生鳍条有关。该品系为进一步研究斑马鱼耳泡的形成和骨骼的发育与再生提供了必要的工具。
We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into 1 cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays post-amputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton.
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影响因子：
14.9
作者：
Warming S;Costantino N;Court DL;Jenkins NA;Copeland NG
通讯作者：
Copeland NG
影响因子：
64.5
作者：
Nakashima, K;Zhou, X;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
14.9
作者：
Flicek P;Aken BL;Beal K;Ballester B;Caccamo M;Chen Y;Clarke L;Coates G;Cunningham F;Cutts T;Down T;Dyer SC;Eyre T;Fitzgerald S;Fernandez-Banet J;Gräf S;Haider S;Hammond M;Holland R;Howe KL;Howe K;Johnson N;Jenkinson A;Kähäri A;Keefe D;Kokocinski F;Kulesha E;Lawson D;Longden I;Megy K;Meidl P;Overduin B;Parker A;Pritchard B;Prlic A;Rice S;Rios D;Schuster M;Sealy I;Slater G;Smedley D;Spudich G;Trevanion S;Vilella AJ;Vogel J;White S;Wood M;Birney E;Cox T;Curwen V;Durbin R;Fernandez-Suarez XM;Herrero J;Hubbard TJ;Kasprzyk A;Proctor G;Smith J;Ureta-Vidal A;Searle S
通讯作者：
Searle S
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
4.6
作者：
Hammond, Christina Lindsey;Schulte-Merker, Stefan
通讯作者：
Schulte-Merker, Stefan