Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse.
Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse.
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DOI：
10.1186/1754-6834-4-40
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发表时间：
2011-10-18
影响因子：
6.3
通讯作者：
Goldman GH
中科院分区：
文献类型：
--
作者：
de Souza WR;de Gouvea PF;Savoldi M;Malavazi I;de Souza Bernardes LA;Goldman MHS;de Vries RP;de Castro Oliveira JV;Goldman GH
考虑到纤维素酶和半纤维素酶的成本对生物乙醇的价格有很大贡献，旨在理解和提高纤维素酶效率和生产力的新研究是至关重要的。尼日尔已被证明产生广谱的多糖水解酶。为了了解如何改善可以水解预处理甘蔗渣的酶混合物，我们使用基因组学方法来研究在A.蒸汽爆破甘蔗渣（SEB）的尼日尔。在这里，我们报告的主要纤维素酶和半纤维素酶编码基因的增加，在SEB生长过程中的表达。我们还试图确定是否几个SEB诱导的基因编码推定的转运蛋白的mRNA积累诱导木糖和依赖于葡萄糖。共鉴定出18株（占A.尼日尔预测的纤维素酶）和21株（A.尼日尔预测半纤维素酶）纤维素酶和半纤维素酶编码基因分别在SEB上生长期间高度表达。甘蔗渣的降解需要产生许多不同的酶，这些酶由可用底物的类型和复杂性调节。我们目前报道的工作为理解甘蔗生物质糖化A。尼日尔水解酶，并用于构建用于第二代生物乙醇的更有效的酶混合物。
Considering that the costs of cellulases and hemicellulases contribute substantially to the price of bioethanol, new studies aimed at understanding and improving cellulase efficiency and productivity are of paramount importance. Aspergillus niger has been shown to produce a wide spectrum of polysaccharide hydrolytic enzymes. To understand how to improve enzymatic cocktails that can hydrolyze pretreated sugarcane bagasse, we used a genomics approach to investigate which genes and pathways are transcriptionally modulated during growth of A. niger on steam-exploded sugarcane bagasse (SEB). Herein we report the main cellulase- and hemicellulase-encoding genes with increased expression during growth on SEB. We also sought to determine whether the mRNA accumulation of several SEB-induced genes encoding putative transporters is induced by xylose and dependent on glucose. We identified 18 (58% of A. niger predicted cellulases) and 21 (58% of A. niger predicted hemicellulases) cellulase- and hemicellulase-encoding genes, respectively, that were highly expressed during growth on SEB. Degradation of sugarcane bagasse requires production of many different enzymes which are regulated by the type and complexity of the available substrate. Our presently reported work opens new possibilities for understanding sugarcane biomass saccharification by A. niger hydrolases and for the construction of more efficient enzymatic cocktails for second-generation bioethanol.
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影响因子：
4.4
作者：
Nakari-Setala, Tiina;Paloheimo, Marja;Saloheimo, Markku
通讯作者：
Saloheimo, Markku
影响因子：
3
作者：
Marui, J;Tanaka, A;Tsukagoshi, N
通讯作者：
Tsukagoshi, N
影响因子：
5
作者：
Maeda, H;Sano, M;Iguchi, Y
通讯作者：
Iguchi, Y
影响因子：
9
作者：
de Cerqueira Leite, Rogerio Cezar;Lima Verde Leal, Manoel Regis;Gaya Scandiffio, Mirna Ivonne
通讯作者：
Gaya Scandiffio, Mirna Ivonne
影响因子：
6.3
作者：
Goldemberg, Jose
通讯作者：
Goldemberg, Jose