A flavonoid 3-O-glucoside:2"-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana.
A flavonoid 3-O-glucoside:2"-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana.
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DOI：
10.1111/tpj.12580
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Saito K
中科院分区：
文献类型：
--
作者：
Yonekura-Sakakibara K;Nakabayashi R;Sugawara S;Tohge T;Ito T;Koyanagi M;Kitajima M;Takayama H;Saito K
黄酮醇3-O-二葡萄糖苷具有1-→-2糖苷键，是广泛存在于植物中的具有代表性的花粉专一性黄酮醇，但其生物合成基因和生理作用尚不清楚。对拟南芥花粉和绒毡层正常发育缺陷的4个花器官(雌蕊、雄蕊、花瓣和花萼)以及野生型和雄性不育突变体Ms1的花进行的类黄酮分析表明，山奈酚/栎素3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranosides在拟南芥花粉中积累。利用野生型和MS1突变体的基因芯片数据，不同器官中的基因表达模式，以及UDP-糖基转移酶(UGTS)的系统发育分析表明，UGT79B6(At5g54010)是决定花粉特异性黄酮醇结构的关键修饰酶。在两个独立的ugt79b6基因敲除突变体中，山奈酚和栎素3-O-葡萄糖基-(1-→，2)-葡萄糖苷缺失。转UGT79B6基因的转基因突变株具有与野生型植株相同的类黄酮谱。重组UGT79B6蛋白将山奈酚3-O-葡萄糖苷转化为山奈酚3-O-葡萄糖-(1-→，2)-葡萄糖苷。UGT79B6可识别3-O-葡萄糖化/半乳糖化的花青素/黄酮醇，但不识别3，5-或3，7-二糖基化的黄酮类化合物，并偏爱UDP-葡萄糖，表明UGT79B6编码类黄酮类3-O-葡萄糖苷：2“-O-葡萄糖基转移酶。UGT79B6-GUS融合结果表明，UGT79B6定位于花药发育过程中的绒毡层细胞和小孢子中。
Flavonol 3-O-diglucosides with a 1→2 inter-glycosidic linkage are representative pollen-specific flavonols that are widely distributed in plants, but their biosynthetic genes and physiological roles are not well understood. Flavonoid analysis of four Arabidopsis floral organs (pistils, stamens, petals and calyxes) and flowers of wild-type and male sterility 1 (ms1) mutants, which are defective in normal development of pollen and tapetum, showed that kaempferol/quercetin 3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranosides accumulated in Arabidopsis pollen. Microarray data using wild-type and ms1 mutants, gene expression patterns in various organs, and phylogenetic analysis of UDP-glycosyltransferases (UGTs) suggest that UGT79B6 (At5g54010) is a key modification enzyme for determining pollen-specific flavonol structure. Kaempferol and quercetin 3-O-glucosyl-(1→2)-glucosides were absent from two independent ugt79b6 knockout mutants. Transgenic ugt79b6 mutant lines transformed with the genomic UGT79B6 gene had the same flavonoid profile as wild-type plants. Recombinant UGT79B6 protein converted kaempferol 3-O-glucoside to kaempferol 3-O-glucosyl-(1→2)-glucoside. UGT79B6 recognized 3-O-glucosylated/galactosylated anthocyanins/flavonols but not 3,5- or 3,7-diglycosylated flavonoids, and prefers UDP-glucose, indicating that UGT79B6 encodes flavonoid 3-O-glucoside:2″-O-glucosyltransferase. A UGT79B6-GUS fusion showed that UGT79B6 was localized in tapetum cells and microspores of developing anthers.
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3.1
作者：
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