Features of Ppd-B1 expression regulation and their impact on the flowering time of wheat near-isogenic lines.
Features of Ppd-B1 expression regulation and their impact on the flowering time of wheat near-isogenic lines.
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DOI：
10.1186/s12870-017-1126-z
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发表时间：
2017-11-14
影响因子：
5.3
通讯作者：
Salina EA
中科院分区：
文献类型：
--
作者：
Kiseleva AA;Potokina EK;Salina EA
光周期不敏感的Ppd-1a等位基因决定小麦的早花。同源异型Ppd-D1 a和Ppd-A1 a的表达增加是由于启动子区的缺失，而Ppd-B1 a的表达增加是由拷贝数增加决定的。本研究以开花期不同的面包小麦品种索诺拉和PSL 2为材料，以索诺拉的Ppd-B1 a基因为材料，利用它们的近等基因系“Ppd-m”和“Ppd-w”，研究了影响Ppd-B1 a基因表达的可能因素。通过分析Ppd-B1 a三个不同的拷贝，我们确定了一个indel和两个SNP，这将所研究的等位基因与具有拷贝数变异的其他等位基因区分开来。我们研究了Ppd-A1、Ppd-B1 a和Ppd-D1基因沿着与光感知相关基因（PhyA、PhyB、PhyC）和开花起始相关基因（Vrn-1、TaFT 1）的表达，并讨论了它们之间的相互作用。在比“Ppd-w”提前四天开花的“Ppd-m”系中，Ppd-B1 a的表达显著更高。我们发现PhyC在具有Ppd-B1 a等位基因的品系中上调。PhyC在“Ppd-m”中的表达量较高。微卫星基因分型结果表明，在“Ppd-m”中，早花亲本索诺拉的5 B染色体近着丝粒区存在基因渐渗现象，而“Ppd-w”中不存在这种基因渐渗现象。FHY 3/FAR 1已知位于该区域。转录因子FHY 3/FAR 1在“Ppd-m”系中的表达高于“Ppd-w”系，表明FHY 3/FAR 1对小麦开花时间有重要影响，可能导致“Ppd-m”系比“Ppd-w”系提早开花。我们提出，有一个积极的双向调节Ppd-B1 a和PhyC与FHY 3/FAR 1的贡献。Ppd-A1 a和Ppd-D1 a可以提出双向调节。使用计算机模拟分析，我们证明了Ppd-B1调节的特异性与同源基因的特异性相比，不仅涉及拷贝数的变化，而且还涉及不同的调节元件。本文的在线版本（10.1186/s12870-017-1126-z）包含补充材料，可供授权用户使用。
Photoperiod insensitive Ppd-1a alleles determine early flowering of wheat. Increased expression of homoeologous Ppd-D1a and Ppd-A1a result from deletions in the promoter region, and elevated expression of Ppd-B1a is determined by an increased copy number. In this study, using bread wheat cultivars Sonora and PSL2, which contrast in flowering time, and near-isogenic lines resulting from their cross, “Ppd-m” and “Ppd-w” with Ppd-B1a introgressed from Sonora, we investigated the putative factors that influence Ppd-B1a expression. By analyzing the Ppd-B1a three distinct copies, we identified an indel and the two SNPs, which distinguished the investigated allele from other alleles with a copy number variation. We studied the expression of the Ppd-A1, Ppd-B1a, and Ppd-D1 genes along with genes that are involved in light perception (PhyA, PhyB, PhyC) and the flowering initiation (Vrn-1, TaFT1) and discussed their interactions. Expression of Ppd-B1a in the “Ppd-m” line, which flowered four days earlier than “Ppd-w”, was significantly higher. We found PhyC to be up-regulated in lines with Ppd-B1a alleles. Expression of PhyC was higher in “Ppd-m”. Microsatellite genotyping demonstrated that in the line “Ppd-m”, there is an introgression in the pericentromeric region of chromosome 5B from the early flowering parental Sonora, while the “Ppd-w” does not have this introgression. FHY3/FAR1 is known to be located in this region. Expression of the transcription factor FHY3/FAR1 was higher in the “Ppd-m” line than in “Ppd-w”, suggesting that FHY3/FAR1 is important for the wheat flowering time and may cause earlier flowering of “Ppd-m” as compared to “Ppd-w”. We propose that there is a positive bidirectional regulation of Ppd-B1a and PhyC with an FHY3/FAR1 contribution. The bidirectional regulation can be proposed for Ppd-A1a and Ppd-D1a. Using in silico analysis, we demonstrated that the specificity of the Ppd-B1 regulation compared to that of homoeologous genes involves not only a copy number variation but also distinct regulatory elements. The online version of this article (10.1186/s12870-017-1126-z) contains supplementary material, which is available to authorized users.
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影响因子：
4.5
作者：
Genoud, Thierry;Schweizer, Fabian;Tscheuschler, Anke;Debrieux, Dimitry;Casal, Jorge J.;Schaefer, Eberhard;Hiltbrunner, Andreas;Fankhauser, Christian
通讯作者：
Fankhauser, Christian
影响因子：
7.2
作者：
BORELLO, U;CECCARELLI, E;GIULIANO, G
通讯作者：
GIULIANO, G
DOI：
10.1073/pnas.1316278111
发表时间：
2014-02-11
影响因子：
11.1
作者：
Franco-Zorrilla, Jose M.;Lopez-Vidriero, Irene;Solano, Roberto
通讯作者：
Solano, Roberto
影响因子：
9.4
作者：
Guo, Zhiai;Song, Yanxia;Jia, Jizeng
通讯作者：
Jia, Jizeng
影响因子：
44.1
作者：
Hu, YX;Wang, YH;Li, JY
通讯作者：
Li, JY