Non-FcεR bearing mast cells secrete sufficient interleukin-4 to control Francisella tularensis replication within macrophages.
Non-FcεR bearing mast cells secrete sufficient interleukin-4 to control Francisella tularensis replication within macrophages.
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DOI：
10.1016/j.cyto.2011.04.009
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发表时间：
2011-08
期刊：
影响因子：
3.8
通讯作者：
Arulanandam, Bernard P.
中科院分区：
文献类型：
--
作者：
Thathiah, Prea;Sanapala, Shilpa;Rodriguez, Annette R.;Yu, Jieh-Juen;Murthy, Ashlesh K.;Guentzel, M. Neal;Forsthuber, Thomas G.;Chambers, James P.;Arulanandam, Bernard P.
肥大细胞通常与过敏和过敏反应的触发有关。然而，最近的研究结果已经阐明了这些细胞选择性地释放各种细胞因子的能力，从而导致通过中性粒细胞和树突状细胞动员的细菌清除，并表明在先天性宿主防御中的重要作用。本实验室建立了原代骨髓源性肥大细胞-巨噬细胞共培养体系，发现肥大细胞通过接触和分泌产物白细胞介素-4（IL-4）介导对土拉弗朗西斯菌LVS在巨噬细胞内摄取和复制的显著抑制。在本研究中，我们利用P815肥大细胞和J774巨噬细胞进一步研究由非Fc εR驱动的信号激活肥大细胞是否可以产生IL-4并控制巨噬细胞内LVS复制。在通过肥大细胞活化肽MP 7活化后收集的P815上清液，以及与J774巨噬细胞共培养的P815细胞，表现出对细菌摄取和复制的显著抑制，这与IL-4的产生相关。在体外观察到的抑制是可滴定的，并且在肺激发后以与细胞浸润事件相关的比率保持。总的来说，我们的数据表明，原代肥大细胞和P815肥大细胞（缺乏FcεR）分泌的IL-4可以控制巨噬细胞内弗朗西斯菌的复制。
Mast cells have classically been implicated in the triggering of allergic and anaphylactic reactions. However, recent findings have elucidated the ability of these cells to selectively release a variety of cytokines leading to bacterial clearance through neutrophil and dendritic cell mobilization, and suggest an important role in innate host defenses. Our laboratory has established a primary bone marrow derived mast cell-macrophage co-culture system and found that mast cells mediated a significant inhibition of Francisella tularensis LVS uptake and replication within macrophages through contact and the secreted product interleukin-4 (IL-4). In this study, we utilized P815 mast cells and J774 macrophages to further investigate whether mast cell activation by non-FcεR driven signals could produce IL-4 and control intramacrophage LVS replication. P815 supernatants collected upon activation by the mast cell activating peptide MP7, as well as P815 cells co-cultured with J774 macrophages, exhibited marked inhibition of bacterial uptake and replication, which correlated with the production of IL-4. The inhibition noted in vitro was titratable and preserved at ratios relevant to cellular infiltration events following pulmonary challenge. Collectively, our data suggest that both primary mast cell and P815 mast cell (lacking FcεR) secreted IL-4 can control intramacrophage Francisella replication.
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影响因子：
64.8
作者：
GORDON, JR;GALLI, SJ
通讯作者：
GALLI, SJ
影响因子：
3.1
作者：
Golovliov, I;Baranov, V;Sjöstedt, A
通讯作者：
Sjöstedt, A
DOI：
10.1006/bbrc.1996.0304
发表时间：
1996-02-27
影响因子：
3.1
作者：
Furuno, T;Teshima, R;Nakanishi, M
通讯作者：
Nakanishi, M
影响因子：
3.1
作者：
Collazo, Carmen M.;Meierovics, Anda I.;Elkins, Karen L.
通讯作者：
Elkins, Karen L.
影响因子：
32.4
作者：
Bird, JJ;Brown, DR;Reiner, SL
通讯作者：
Reiner, SL