High-resolution association mapping of number of teats in pigs reveals regions controlling vertebral development.
High-resolution association mapping of number of teats in pigs reveals regions controlling vertebral development.
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DOI：
10.1186/1471-2164-15-542
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发表时间：
2014-06-30
期刊：
影响因子：
4.4
通讯作者：
Harlizius B
中科院分区：
文献类型：
--
作者：
Duijvesteijn N;Veltmaat JM;Knol EF;Harlizius B
对乳头数量施加选择压力，以便能够提供足够的乳头来增加猪的窝产仔数。虽然已经报道了许多QTL，但它们覆盖了大的染色体区域，功能突变及其潜在的生物学机制尚未确定。为了更好地了解乳头性状数量的遗传结构，我们使用Illumina PorcineSNP 60 Beadchip对936头大白色猪进行了基因分型，进行了全基因组关联研究。该分析是基于回归育种值，占密集的家庭结构和贝叶斯方法估计的SNP的影响。全基因组关联研究产生了212个显著的SNP。在13条猪染色体上共定位了39个QTL区域，包括170个SNP位点，其中位于SSC 7、9、10、12和14上的5个QTL区域达到极显著水平。所有显著相关的区域一起解释了9.5%的遗传方差，其中SSC 7上的QTL解释了最多的遗传方差（2.5%）。对于五个高度显著的QTL区域，进行候选基因的搜索。最有说服力的候选基因是SSC 7上的VRTN和Prox 2，SSC 10上的MPP 7、ARMC 4和MKX，以及SSC 12上的椎骨δ-EF 1。所有三个QTL都含有已知与脊椎发育相关的候选基因。在SSC 9和SSC 14上的新QTL区域中，没有发现明显的候选基因。在SSC 7、9、10、12和14上发现了5个主效QTL，其中位于SSC 9和SSC 14上的QTL是首次在这些染色体上发现的。本研究中发现的显著SNPs可用于选择，以增加猪的乳头数量，从而使母猪可以喂养越来越多的活产仔猪。这项研究指出了调节椎骨数量和乳头数量的共同遗传机制。本文的在线版本（doi：10.1186/1471-2164-15-542）包含补充材料，可供授权用户使用。
Selection pressure on the number of teats has been applied to be able to provide enough teats for the increase in litter size in pigs. Although many QTL were reported, they cover large chromosomal regions and the functional mutations and their underlying biological mechanisms have not yet been identified. To gain a better insight in the genetic architecture of the trait number of teats, we performed a genome-wide association study by genotyping 936 Large White pigs using the Illumina PorcineSNP60 Beadchip. The analysis is based on deregressed breeding values to account for the dense family structure and a Bayesian approach for estimation of the SNP effects. The genome-wide association study resulted in 212 significant SNPs. In total, 39 QTL regions were defined including 170 SNPs on 13 Sus scrofa chromosomes (SSC) of which 5 regions on SSC7, 9, 10, 12 and 14 were highly significant. All significantly associated regions together explain 9.5% of the genetic variance where a QTL on SSC7 explains the most genetic variance (2.5%). For the five highly significant QTL regions, a search for candidate genes was performed. The most convincing candidate genes were VRTN and Prox2 on SSC7, MPP7, ARMC4, and MKX on SSC10, and vertebrae δ-EF1 on SSC12. All three QTL contain candidate genes which are known to be associated with vertebral development. In the new QTL regions on SSC9 and SSC14, no obvious candidate genes were identified. Five major QTL were found at high resolution on SSC7, 9, 10, 12, and 14 of which the QTL on SSC9 and SSC14 are the first ones to be reported on these chromosomes. The significant SNPs found in this study could be used in selection to increase number of teats in pigs, so that the increasing number of live-born piglets can be nursed by the sow. This study points to common genetic mechanisms regulating number of vertebrae and number of teats. The online version of this article (doi:10.1186/1471-2164-15-542) contains supplementary material, which is available to authorized users.
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影响因子：
2.9
作者：
Duijvesteijn N;Knol EF;Merks JW;Crooijmans RP;Groenen MA;Bovenhuis H;Harlizius B
通讯作者：
Harlizius B
影响因子：
2.4
作者：
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通讯作者：
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影响因子：
2.5
作者：
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影响因子：
2.9
作者：
Ding, Nengshui;Guo, Yuanmei;Huang, Lusheng
通讯作者：
Huang, Lusheng
影响因子：
3.5
作者：
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通讯作者：
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