The impact of in vitro cultivation on the natural life cycle of the tick-borne relapsing fever spirochete Borrelia turicatae.
The impact of in vitro cultivation on the natural life cycle of the tick-borne relapsing fever spirochete Borrelia turicatae.
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DOI：
10.1371/journal.pone.0239089
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Lopez JE
中科院分区：
文献类型：
--
作者：
Krishnavajhala A;Armstrong BA;Lopez JE
蜱传回归热是一种由疏螺旋体属引起的传染病，主要由鸟虱属蜱传播。先前的工作表明，在体外培养的螺旋体仍然通过针接种对小鼠具有感染性;然而，实验室繁殖对病原体自然生命周期的影响尚未确定。我们目前的研究评估了连续培养对自然蜱哺乳动物传播周期的影响。首先，我们评估了来自B的基因组DNA图谱。turicelae生长到30、60、120和300代，并且这些螺旋体被用于针刺小鼠。未感染的蜱若虫喂养这些小鼠和收购，transstadial维护，并随后确定蜱叮咬后的传播。以定植有B的蜱虫喂养的小鼠中的感染频率。传至30、60和120代的虫口率分别为100%、100%和30%。在120代之后，没有检测到通过蜱喂养的小鼠的成功感染。量化B。蜱组织中的turiclycin表明，到300代时，它们不再定殖于载体。结果表明，体外培养显着影响蜱定植和小鼠感染的建立。这项工作为确定蜱-哺乳动物感染周期中的基本遗传元件提供了基础。
Tick-borne relapsing fever is an infectious disease caused by Borrelia species and are primarily transmitted by Ornithodoros ticks. Prior work indicated that in vitro cultivated spirochetes remain infectious to mice by needle inoculation; however, the impact of laboratory propagation on the pathogens natural life cycle has not been determined. Our current study assessed the effect of serial cultivation on the natural tick-mammalian transmission cycle. First, we evaluated genomic DNA profiles from B. turicatae grown to 30, 60, 120, and 300 generations, and these spirochetes were used to needle inoculate mice. Uninfected nymphal ticks were fed on these mice and acquisition, transstadial maintenance, and subsequent transmission after tick bite was determined. Infection frequencies in mice that were fed upon by ticks colonized with B. turicatae grown to 30, 60, and 120 generations were 100%, 100%, and 30%, respectively. Successful infection of mice by tick feeding was not detected after 120 generations. Quantifying B. turicatae in tick tissues indicated that by 300 generations they no longer colonized the vector. The results indicate that in vitro cultivation significantly affects the establishment of tick colonization and murine infection. This work provides a foundation for the identification of essential genetic elements in the tick-mammalian infectious cycle.
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影响因子：
9.4
作者：
BARBOUR, AG
通讯作者：
BARBOUR, AG
影响因子：
2.1
作者：
Lopez, Job E.;Schrumpf, Merry E.;Schwan, Tom G.
通讯作者：
Schwan, Tom G.
影响因子：
4.4
作者：
Krishnavajhala, Aparna;Wilder, Hannah K.;Lopez, Job E.
通讯作者：
Lopez, Job E.
影响因子：
4.4
作者：
Krishnavajhala, Aparna;Armstrong, Brittany A.;Lopez, Job E.
通讯作者：
Lopez, Job E.
影响因子：
3.2
作者：
Elbir, Haitham;Gimenez, Gregory;Drancourt, Michel
通讯作者：
Drancourt, Michel