Activin inhibits binding of transcription factor Pit-1 to the growth hormone promoter.
Activin inhibits binding of transcription factor Pit-1 to the growth hormone promoter.
复制标题
Activin 抑制转录因子 Pit-1 与生长激素启动子的结合。
DOI：
10.1073/pnas.89.23.11451
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发表时间：
1992
影响因子：
11.1
通讯作者：
Vale,WW
中科院分区：
文献类型：
--
作者：
Struthers,RS;Gaddy-Kurten,D;Vale,WW
激活素A是一种与转化生长因子β相关的强效生长分化因子。在生长激素中，激活素抑制生长激素（GH）的生物合成和分泌以及细胞增殖。我们在这里报道，在MtTW15生长营养肿瘤细胞中，激活素降低了GH mRNA水平，抑制了转染的GH启动子-氯霉素乙酰转移酶融合基因的表达。介导这种抑制的核苷酸序列的缺失定位导致鉴定出先前被表征为结合垂体特异性转录因子Pit-1/GHF-1的区域。核因子与该区域结合的表征表明，激活素处理后，Pit-1与GH启动子的结合丢失。这些结果表明激活素诱导的生长激素生物合成的抑制是由与生长激素启动子结合的组织特异性转录因子的缺失介导的，并提示了其他激活素反应的可能的一般机制，其中激活素调节其他含有POU或同源域的转录因子的功能。
Activin A is a potent growth and differentiation factor related to transforming growth factor beta. In somatotrophs, activin suppresses the biosynthesis and secretion of growth hormone (GH) and cellular proliferation. We report here that, in MtTW15 somatotrophic tumor cells, activin decreased GH mRNA levels and inhibited expression of transfected GH promoter--chloramphenicol acetyltransferase fusion genes. Deletion mapping of nucleotide sequences mediating this inhibition led to the identification of a region that has previously been characterized as binding the pituitary-specific transcription factor Pit-1/GHF-1. Characterization of nuclear factor binding to this region demonstrated that binding of Pit-1 to the GH promoter is lost on activin treatment. These results indicate that activin-induced repression of GH biosynthesis is mediated by the loss of tissue-specific transcription factor binding to the GH promoter and suggest a possible general mechanism for other activin responses, whereby activin regulates the function of other POU- or homeodomain-containing transcription factors.
离散顺式活性基因组序列决定大鼠催乳素和生长激素基因的垂体细胞类型特异性表达
影响因子：
64.8
作者：
C. Nelson;E. Crenshaw;R. Franco;S. Lira;V. Albert;Ronald M. Evans;Ronald M. Evans;Michael G. Rosenfeld;Michael G. Rosenfeld
通讯作者：
Michael G. Rosenfeld
影响因子：
56.9
作者：
NELSON, C;ALBERT, VR;ROSENFELD, MG
通讯作者：
ROSENFELD, MG
影响因子：
64.8
作者：
CHEN, RP;INGRAHAM, HA;ROSENFELD, MG
通讯作者：
ROSENFELD, MG