Hydrogel-Based Colloidal Polymeric System for Protein and Drug Delivery: Physical and Chemical Characterization, Permeability Control and Applications
Hydrogel-Based Colloidal Polymeric System for Protein and Drug Delivery: Physical and Chemical Characterization, Permeability Control and Applications
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用于蛋白质和药物输送的水凝胶基胶体聚合物系统：物理和化学表征、渗透性控制和应用
DOI：
10.1007/3-540-45362-8_3
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发表时间：
2002
影响因子：
--
通讯作者：
J. Davidson
中科院分区：
文献类型：
--
作者：
A. Prokop;E. Kozlov;G. Carlesso;J. Davidson
聚合物纳米颗粒作为药物载体的应用越来越受到学术界和工业界的关注。开发适合于高分子量和短半衰期的蛋白质药物的递送系统是当前的兴趣。此外，纳米颗粒在药物和疫苗递送以及基因治疗应用中具有许多潜在的应用。这篇文章介绍了一种新的生产技术的纳米粒子组成的多组分聚合物复合物，是候选人的生物分子，如蛋白质和药物的传递载体。材料科学的理论和实践为开发不溶于水和缓冲介质的高度压实结构提供了基础。生物相容性和大多数天然聚合物在不存在有机溶剂的情况下，使用两种类型的加工方法制成化学稳定的纳米颗粒：分批和连续。仔细选择建筑材料和在生产过程中叠加几种相互作用的原理，可以定制结构的物理化学性质。在用于组装纳米颗粒的典型聚合物中，研究了不同的多糖、天然胺和多胺。测试的包埋物质包括蛋白质、抗原和小药物分子。纳米粒子的大小和电荷被认为是在生物系统中应用的首要问题。在批处理和连续系统中的详细实验允许生产过程的时间依赖性化学计量表征和对这种超分子结构的基本组装原理的理解。连续流生产显示出在产品质量和一致性方面提供更一致的数据，具有进一步的工艺开发和商业化的可能性。为了控制渗透性，将聚葡聚糖醛掺入到颗粒核心中，以使生理交联和长期保留的物质，否则将迅速泄漏出纳米颗粒。交联实验的结果清楚地表明，释放速率可以大大降低，这取决于交联的程度。对于疫苗抗原递送试验，我们测量了皮下和口服给药后的抗体产生。数据表明，当使用口服给药途径时，仅交联抗原具有免疫原性。本文中提出的数据主要涉及纳米颗粒用于口服递送疫苗抗原的效用。这种新技术与其他技术（主要是基于水和有机溶剂的技术）相比，得到了广泛的讨论。使用几个例子，评估蛋白质和小的药物输送的有用性。
The use of polymeric nanoparticles as drug carriers is receiving an increasing amount of attention both in academia and industry. The development of suitable delivery systems for protein drugs with high molecular weights and short half-lives is of current interest. In addition, nanoparticles have a number of potential applications in drug and vaccine delivery as well as gene therapy applications. This article features a new production technology for nanoparticles comprised of multicomponent polymeric complexes that are candidates for delivery vehicles of biological molecules such as proteins and drugs. Materials science theory and practice provide the basis for the development of highly compacted structures that are insoluble in water and buffered media. Biocompatible and mostly natural polymers are fabricated into thermodynamically stable nanoparticles, in the absence of organic solvents, using two types of processing: batch and continuous. Careful choice of construction materials and the superposition of several interacting principles during their production allow for the customization of the physicochemical properties of the structures. Among the typical polymers used to assemble nanoparticles, different polysaccharides, natural amines and polyamines were investigated. The entrapped substances tested included proteins, antigens and small drug molecules. The size and charge of nanoparticles is considered to be of primary importance for application in biological systems. Detailed experiments in batch and continuous systems allowed time-dependent stoichiometric characterization of the production process and an understanding of fundamental assembly principles of such supramolecular structures. Continuous-flow production is shown to provide more consistent data in terms of product quality and consistency, with further possibilities of process development and commercialization. To control permeability, polydextran aldehyde, incorporated into the particle core, was used to enable physiologic cross-linking and long-term retention of substances that would otherwise rapidly leak out of the nanoparticles. Results of cross-linking experiments clearly demonstrated that the release rate could be substantially reduced, depending on the degree of cross-linking. For vaccine antigen delivery tests we measured an antibody production following subcutaneous and oral administration. The data indicated that only the cross-linked antigen was immunogenic when the oral route of administration was used. The data presented in this paper address primarily the utility of nanoparticulates for oral delivery of vaccine antigen. This novel technology is extensively discussed in contrast to other technologies, primarily water- and organic solvent-based. The usefulness is demonstrated using several examples, evaluating protein and small drug delivery.
DOI：
--
发表时间：
1992-06
期刊：
The American journal of pathology
影响因子：
--
作者：
G. Pierce;J. Tarpley;D. Yanagihara;Thomas A. Mustoe, MD, FACS;G. M. Fox;A. Thomason
通讯作者：
G. Pierce;J. Tarpley;D. Yanagihara;Thomas A. Mustoe, MD, FACS;G. M. Fox;A. Thomason
用于蛋白质递送的水基纳米颗粒聚合物系统：渗透性控制和疫苗应用。
影响因子：
3.8
作者：
Prokop,Ales;Kozlov,Evgenii;Newman,GaleW;Newman,MarkJ
通讯作者：
Newman,MarkJ
一种使用 Iodobeads 跟踪蛋白质放射性碘化时间过程的膜印迹方法。
影响因子：
2.9
作者：
Cheng,H;Rudick,MJ
通讯作者：
Rudick,MJ