Porcine reproductive and respiratory syndrome virus neutralizing antibodies provide in vivo cross-protection to PRRSV1 and PRRSV2 viral challenge.
Porcine reproductive and respiratory syndrome virus neutralizing antibodies provide in vivo cross-protection to PRRSV1 and PRRSV2 viral challenge.
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DOI：
10.1016/j.virusres.2018.01.015
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发表时间：
2018-03-15
期刊：
影响因子：
5
通讯作者：
Murtaugh MP
中科院分区：
文献类型：
--
作者：
Robinson SR;Rahe MC;Gray DK;Martins KV;Murtaugh MP
猪繁殖与呼吸综合征（PRRS）是猪最重要的疾病，对其进行疫苗防控很难实现。然而，在典型的田间条件下发现的针对猪繁殖与呼吸综合征病毒（PRRSV）的广泛中和抗体活性，为实现强有力保护的新型免疫学方法打开了大门。我们在此表明，被动给予含中和抗体的纯化免疫球蛋白可使PRRSV2感染降低多达96%，使PRRSV1感染降低多达87%，而缺乏中和活性的免疫球蛋白对病毒感染没有影响。因此，被动免疫球蛋白转移的免疫能力与抗体中和活性有特定关联。当前的PRRSV感染模型表明，一种包含GP2、GP3和GP4的次要包膜糖蛋白（GP）复合物对允许细胞感染至关重要。然而，构成假定细胞附着结构的保守肽段并没有减弱中和作用或病毒感染。结果表明，旨在诱导广泛中和抗体的免疫学方法可能会大大增强对PRRSV的免疫保护。这些发现还表明，自然发生的病毒分离株能够诱导针对不相关的PRRSV攻击的保护性体液免疫，从而消除了疫苗研发的一个主要概念障碍。
Vaccine control and prevention of porcine reproductive and respiratory syndrome (PRRS), the most important disease of swine, is difficult to achieve. However, the discovery of broadly neutralizing antibody activity against porcine reproductive and respiratory syndrome virus (PRRSV) under typical field conditions opens the door to new immunologic approaches for robust protection. We show here that passive administration of purified immunoglobulins with neutralizing antibodies reduced PRRSV2 infection by up to 96%, and PRRSV1 infection by up to 87%, whereas immune immunoglobulins lacking neutralizing activity had no effect on viral infection. Hence, immune competence of passive immunoglobulin transfer was associated specifically with antibody neutralizing activity. Current models of PRRSV infection implicate a minor envelope glycoprotein (GP) complex including GP2, GP3, and GP4, as critical to permissive cell infection. However, conserved peptides comprising the putative cell attachment structure did not attenuate neutralization or viral infection. The results show that immunological approaches aimed at induction of broadly neutralizing antibodies may substantially enhance immune protection against PRRSV. The findings further show that naturally occurring viral isolates are able to induce protective humoral immunity against unrelated PRRSV challenge, thus removing a major conceptual barrier to vaccine development.
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