The clock component OsLUX regulates rice heading through recruiting OsELF3-1 and OsELF4s to repress Hd1 and Ghd7.
The clock component OsLUX regulates rice heading through recruiting OsELF3-1 and OsELF4s to repress Hd1 and Ghd7.
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时钟组件 OsLUX 通过招募 OsELF3-1 和 OsELF4 抑制 Hd1 和 Ghd7 来调节水稻抽穗
DOI：
10.1016/j.jare.2022.08.001
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发表时间：
2023-06
影响因子：
10.7
通讯作者：
Wu, Weixun
中科院分区：
文献类型：
--
作者：
Xu, Peng;Zhang, Yingxin;Wen, Xiaoxia;Yang, Qinqin;Liu, Ling;Hao, Shulei;Li, Jiaxin;Wu, Zhaozhong;Shah, Liaqat;Sohail, Amir;Liu, Qunen;Sun, Lianping;Hong, Yongbo;Chen, Daibo;Shen, Xihong;Zhan, Xiaodeng;Cheng, Shihua;Cao, Liyong;Wu, Weixun
osux通过抑制Hd1和Ghd7调控水稻抽穗。在两种光周期条件下，缺陷osux导致依赖于Hd1和Ghd7的极晚抽穗表型。细胞质-核穿梭机制解释了OsELF3-1对oslx的影响。完整的OsEC （oself4s - oself3 -1 - oselux）复合体通过结合Hd1和Ghd7的启动子来调节头部。OsEC对Hd1和Ghd7的抑制强度解释了OsEC组分的不同开花效果。生物钟协调内部生理和外部环境因素调节谷物开花，这对生殖生长和最佳产量决定至关重要。在本研究中，我们旨在确认OsLUX在水稻开花时间调控中的作用。进一步的研究阐明了oself4s - oself3 -1 - oselux复合体如何直接调控开花相关基因介导水稻抽穗。我们通过MutMap方法确定了一个昼夜节律基因OsLUX。研究了WT和oslx突变体开花相关基因的转录水平。oselux形成的OsEC （oself4 - oself3 -1 - oselux）复合物通过酵母双杂交、拉下、BiFC和荧光素酶互补试验（LCA）得到支持。通过EMSA、Chip-qPCR、荧光素酶发光图像和相对LUC活性分析来检测OsEC （oself4 - oself3 -1 - oselux）复合物对开花基因的靶向调控。昼夜节律基因osux编码MYB家族转录因子，该转录因子作为重要的昼夜节律时钟调节因子并控制水稻抽穗。OsLUX缺陷在自然长昼和短昼条件下导致极晚的出穗期表型，其功能通过基因互补、过表达和CRISPR/Cas9敲除进一步得到证实。oselux通过招募调控水稻抽穗所需的OsELF3-1和oself4形成OsEC （oself4 - OsELF3-1 - oselux）复合体。OsELF3-1有助于oselux易位到细胞核，并且OsEC复合物的任何组分突变都会导致开花表型受损。OsEC复合体通过结合Hd1和Ghd7启动子的LBS （LUX结合位点）元件直接抑制Hd1和Ghd7的表达。我们的研究结果表明，昼夜节律基因oslx通过直接调控节律振荡和核心开花时间相关基因来调控水稻抽穗。我们揭示了OsEC靶点通过直接抑制Hd1和Ghd7的表达来调节水稻光周期开花的机制。OsEC (oself4s - oself3 -1 - oselux) -Hd1 /Ghd7调控模块为作物改良提供遗传靶点。
OsLUX regulates rice heading by repressing Hd1 and Ghd7. Defect OsLUX causes extremely late heading phenotype dependent on Hd1 and Ghd7 under both photoperiod conditions. The cytoplasm-nuclear shuttling mechanism explains the effect of OsELF3-1 on OsLUX. The complete OsEC (OsELF4s–OsELF3-1–OsLUX) complex is required to regulate heading via binding to the promoters of Hd1 and Ghd7. The repressive strength of OsEC to regulate Hd1 and Ghd7 explained the different flowering effects of OsEC components. Circadian clocks coordinate internal physiology and external environmental factors to regulate cereals flowering, which is critical for reproductive growth and optimal yield determination. In this study, we aimed to confirm the role of OsLUX in flowering time regulation in rice. Further research illustrates how the OsELF4s–OsELF3-1–OsLUX complex directly regulates flowering-related genes to mediate rice heading. We identified a circadian gene OsLUX by the MutMap method. The transcription levels of flowering-related genes were evaluated in WT and oslux mutants. OsLUX forms OsEC (OsELF4s–OsELF3-1–OsLUX) complex were supported by yeast two-hybrid, pull down, BiFC, and luciferase complementation assays (LCA). The EMSA, Chip-qPCR, luciferase luminescence images, and relative LUC activity assays were performed to examine the targeted regulation of flowering genes by the OsEC (OsELF4s–OsELF3-1–OsLUX) complex. The circadian gene OsLUX encodes an MYB family transcription factor that functions as a vital circadian clock regulator and controls rice heading. Defect in OsLUX causes an extremely late heading phenotype under natural long-day and short-day conditions, and the function was further confirmed through genetic complementation, overexpression, and CRISPR/Cas9 knockout. OsLUX forms the OsEC (OsELF4s–OsELF3-1–OsLUX) complex by recruiting OsELF3-1 and OsELF4s, which were required to regulate rice heading. OsELF3-1 contributes to the translocation of OsLUX to the nucleus, and a compromised flowering phenotype results upon mutation of any component of the OsEC complex. The OsEC complex directly represses Hd1 and Ghd7 expression via binding to their promoter's LBS (LUX binding site) element. Our findings show that the circadian gene OsLUX regulates rice heading by directly regulating rhythm oscillation and core flowering-time-related genes. We uncovered a mechanism by which the OsEC target suppresses the expression of Hd1 and Ghd7 directly to modulate photoperiodic flowering in rice. The OsEC (OsELF4s–OsELF3-1–OsLUX)–Hd1/Ghd7 regulatory module provides the genetic targets for crop improvement.
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影响因子：
18
作者：
Ezer D;Jung JH;Lan H;Biswas S;Gregoire L;Box MS;Charoensawan V;Cortijo S;Lai X;Stöckle D;Zubieta C;Jaeger KE;Wigge PA
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.2010241118
发表时间：
2021-02-23
影响因子：
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作者：
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通讯作者：
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