Genetic characterization of the first detected human case of avian influenza A (H5N6) in Anhui Province, East China.
Genetic characterization of the first detected human case of avian influenza A (H5N6) in Anhui Province, East China.
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中国东部安徽省首例发现的人感染甲型H5N6禽流感病例的基因特征
DOI：
10.1038/s41598-018-33356-4
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发表时间：
2018-10-16
影响因子：
4.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
He J;Liu BY;Gong L;Chen Z;Chen XL;Hou S;Yu JL;Wu JB;Xia ZC;Latif A;Gao R;Su B;Liu Y
我们比较了从安徽省一名患者身上分离的两株H5 N6禽流感病毒与GenBank和全球流感数据共享倡议（GISAID）中的其他毒株的全基因组序列。该毒株与A/chicken/Zhejiang/727155/2014（H5 N6）毒株HA基因同源性为98%。安徽分离株的6个内部基因与浙江、山东、广东3省的H9 N2病毒相似性均为99%。此外，分离的H5 N6病毒与H7N9和H10 N8病毒的内部抗原（NP和MP）之间的相似性为99%。系统进化分析结果表明，安徽省H5 N6亚型流感病毒属于2.3.4.4进化枝。该病毒具有高致病性禽流感病毒的分子特征，包括8个糖基化位点和HA蛋白切割位点的氨基酸序列PLRERRKKR/GLF，含有多个碱性氨基酸。此外，发现NA蛋白的茎域在NA茎区（N6中的11个氨基酸，位置58-68）中具有缺失。我们的研究表明，安徽省H5 N6病毒代表了三重病毒，可能对人类具有高致病性。应密切监测这种病毒的流行情况。
We compared complete genome sequences of two strains of an avian influenza A (H5N6) virus isolated from a patient in Anhui Province with those of other strains from GenBank and Global initiative on sharing all influenza data (GISAID). The HA gene of the isolated virus shared homology with that of A/chicken/Zhejiang/727155/2014 (H5N6) at the level of similarity of 98%. The six internal genes of the Anhui strains were close to those of H9N2 viruses from Zhejiang, Shandong, and Guangdong provinces, with a similarity of 99%. In addition, the similarity between the internal antigens (NP and MP) of the isolated H5N6 virus and H7N9 and H10N8 viruses was 99%. Based on the data of phylogenetic analysis, the H5N6 influenza virus isolated in Anhui Province belonged to clade 2.3.4.4. The virus was shown to have molecular characteristics of highly pathogenic avian influenza viruses, including eight glycosylation sites and an amino acid sequence of the HA protein cleavage site, PLRERRRKKR/GLF, containing multiple basic amino acids. Additionally, the stalk domain of the NA protein was found to have a deletion in NA stalk region (11 amino acids in N6, positions 58–68). Our study demonstrated that the H5N6 virus from Anhui Province represented a triple-reassortant virus and could be highly pathogenic to humans. The prevalence of this virus should be closely monitored.
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DOI：
10.1136/bmj.f4752
发表时间：
2013-08-06
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Qi X;Qian YH;Bao CJ;Guo XL;Cui LB;Tang FY;Ji H;Huang Y;Cai PQ;Lu B;Xu K;Shi C;Zhu FC;Zhou MH;Wang H
通讯作者：
Wang H
鸟类流感病毒中神经氨酸酶茎缺失的出现和遗传变异。
影响因子：
3.7
作者：
Li J;Zu Dohna H;Cardona CJ;Miller J;Carpenter TE
通讯作者：
Carpenter TE
影响因子：
3.7
作者：
Fan, Shufang;Deng, Guohua;Chen, Hualan
通讯作者：
Chen, Hualan
DOI：
10.1126/science.1213362
发表时间：
2012-06-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Herfst S;Schrauwen EJ;Linster M;Chutinimitkul S;de Wit E;Munster VJ;Sorrell EM;Bestebroer TM;Burke DF;Smith DJ;Rimmelzwaan GF;Osterhaus AD;Fouchier RA
通讯作者：
Fouchier RA
DOI：
10.1128/genomea.00469-13
发表时间：
2013-07-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Mi, Zhiqiang;Liu, Wei;Tong, Yigang
通讯作者：
Tong, Yigang