Enhanced cross-species utility of conserved microsatellite markers in shorebirds.
Enhanced cross-species utility of conserved microsatellite markers in shorebirds.
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DOI：
10.1186/1471-2164-9-502
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发表时间：
2008-10-24
期刊：
影响因子：
4.4
通讯作者：
Dawson DA
中科院分区：
文献类型：
--
作者：
Küpper C;Burke T;Székely T;Dawson DA
微卫星标记是法医学中常用的遗传标记。尽管他们的普及，多态性微卫星位点的特点和发展合适的标记需要相当大的努力。新的可用的基因组数据库使得鉴定保守的遗传标记成为可能。我们研究的实用性和特点，保守的微卫星标记在Charadriiformes（？，鹬，海鸥和海雀）。该目拥有许多物种，具有不同的繁殖系统，生活史和非凡的迁移生物学，其遗传学值得研究。然而，研究在很大程度上受到遗传标记有限的限制。为了研究保守的微卫星基因座作为遗传标记的效用，我们整理了一个数据库的Charadriiformes微卫星，寻找同源基因在鸡基因组和测试保守的标记扩增和多态性的charadriiformes物种的范围。68（42%）的161 charadriiform微卫星位点被分配到一个单一的位置在鸡基因组中的基础上，其E值。55个引物设计的保守微卫星位点的E-值为E-10或更低的扩增范围更广的charadriiform物种比对照组的引物从10个匿名微卫星位点。24个保守标记中有23个是多态的，平均每个标记在12个物种中的3个。基因组序列数据库是鉴定包括位于非编码区的那些在内的保守遗传标记的有用工具。通过最大化源物种和数据库物种之间的引物序列相似性，可以进一步改进标记，并提供额外的标记来研究非模式生物种群的分子生态学。
Microsatellite markers are popular genetic markers frequently used in forensic biology. Despite their popularity, the characterisation of polymorphic microsatellite loci and development of suitable markers takes considerable effort. Newly-available genomic databases make it feasible to identify conserved genetic markers. We examined the utility and characteristics of conserved microsatellite markers in Charadriiformes (plovers, sandpipers, gulls and auks). This order harbours many species with diverse breeding systems, life histories and extraordinary migration biology whose genetics warrant investigation. However, research has been largely restrained by the limited availability of genetic markers. To examine the utility of conserved microsatellite loci as genetic markers we collated a database of Charadriiformes microsatellites, searched for homologues in the chicken genome and tested conserved markers for amplification and polymorphism in a range of charadriiform species. Sixty-eight (42%) of 161 charadriiform microsatellite loci were assigned to a single location in the chicken genome based on their E-value. Fifty-five primers designed from conserved microsatellite loci with an E-value of E-10 or lower amplified across a wider range of charadriiform species than a control group of primers from ten anonymous microsatellite loci. Twenty-three of 24 examined conserved markers were polymorphic, each in on average 3 of 12 species tested. Genomic sequence databases are useful tools to identify conserved genetic markers including those located in non-coding regions. By maximising primer sequence similarity between source species and database species, markers can be further improved and provide additional markers to study the molecular ecology of populations of non-model organisms.
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影响因子：
4.9
作者：
Akesson, Mikael;Hansson, Bengt;Bensch, Staffan
通讯作者：
Bensch, Staffan
DOI：
10.1111/j.1471-8286.2005.00906.x
发表时间：
2005-06-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Dawson, DA;Hunter, FM;Burke, T
通讯作者：
Burke, T
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
3.3
作者：
Baker, Allan J.;Pereira, Sergio L.;Paton, Tara A.
通讯作者：
Paton, Tara A.
影响因子：
2.4
作者：
Baratti, M;Alberti, A;Fulgheri, FD
通讯作者：
Fulgheri, FD