Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus).
Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus).
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DOI：
10.1002/nbm.3864
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发表时间：
2018-03
影响因子：
2.9
通讯作者：
Silva AC
中科院分区：
文献类型：
--
作者：
Hirano Y;Yen CC;Liu JV;Mackel JB;Merkle H;Nascimento GC;Stefanovic B;Silva AC
了解脑刺激下血流动力学反应函数（HRF）的时空特征，对于正确应用神经影像学方法研究脑功能至关重要。在这里，我们研究了有意识和清醒的狨猴（Callithrix jacchus）的血氧水平依赖（BOLD）和脑血容量（CBV） HRF的时空演变。狨猴是一种新世界的非人类灵长类动物，具有无脑脑，在生物医学研究中越来越多地使用。狨猴适应头部固定并放置在7T MRI中。体感刺激（333 μs脉冲，2 mA振幅，64 Hz）通过一对接触电极双向传递。在块设计范式中，刺激持续时间以伪随机顺序增加，从一个脉冲到256个电脉冲（4 s）。对于CBV测量，采用静脉注射30 mg/kg氧化铁纳米颗粒（MION）。在所有刺激条件下，S1、S2和尾状核均获得了稳健的BOLD和CBV hrf。其中，BOLD和CBV对单个333 μs长的刺激反应较为可靠，CBV HRF的起效时间和峰值时间均短于BOLD HRF。随着刺激持续时间的增加，激活区域的大小和峰值幅度都迅速增长，刺激持续时间大于15时达到饱和。S1和S2的发病时间比尾状核快。最后，醒着的狨猴HRF的精细时空特征与人类相似，这表明继续完善醒着的非人类灵长类动物模型对于最大限度地提高动物fMRI研究对人类大脑功能研究的适用性至关重要。我们研究了有意识和清醒的狨猴对不同持续时间的双侧手腕刺激的BOLD和CBV fMRI反应的时空演变。在333 μs的单次刺激下，BOLD和CBV的反应是可靠的，CBV HRF的起效时间和峰值时间都较短。随着刺激持续时间的增加，激活区域的大小和峰值幅度均迅速增大，刺激持续时间大于15时达到饱和。
Understanding the spatiotemporal features of the hemodynamic response function (HRF) to brain stimulation is essential to the proper application of neuroimaging methods to study brain function. Here, we investigated the spatiotemporal evolution of the blood oxygenation level-dependent (BOLD) and cerebral blood volume (CBV) HRF in conscious, awake marmosets (Callithrix jacchus), a New World nonhuman primate with a lissencephalic brain and with growing use in biomedical research. The marmosets were acclimated to head fixation and placed in a 7T MRI. Somatosensory stimulation (333 μs pulses, 2 mA amplitude, 64 Hz) was delivered bilaterally via pairs of contact electrodes. A block design paradigm was used in which the stimulus duration increased in pseudo-random order from a single pulse up to 256 electrical pulses (4 s). For CBV measurements, 30 mg/kg of iron oxide nanoparticles (MION) injected intravenously was used. Robust BOLD and CBV HRFs were obtained in S1, S2, and caudate at all stimuli conditions. In particular, BOLD and CBV responses to a single 333-μs-long stimulus were reliably measured, and the CBV HRF presented shorter onset time and time-to-peak than the BOLD HRF. Both the size of the regions of activation and the peak amplitude of the HRFs grew quickly with increasing stimulus duration, and saturated for stimulus durations greater than 1s. Onset times in S1 and S2 were faster than in caudate. Finally, the fine spatiotemporal features of the HRF in awake marmosets were similar to those obtained in humans, indicating that the continued refinement of awake non-human primate models is essential to maximize the applicability of animal fMRI studies to the investigation of human brain function. We investigated the spatiotemporal evolution of the BOLD and CBV fMRI responses to bilateral wrist stimulation of varying durations in conscious, awake marmosets. BOLD and CBV responses to a single 333-μs-long stimulus were reliably measured, and the CBV HRF presented a shorter onset time and time-to-peak. Both the size of the regions of activation and the peak amplitude of the HRFs grew quickly with stimulus duration, and saturated for stimulus durations greater than 1s.
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影响因子：
2.9
作者：
Papoti, Daniel;Yen, Cecil Chern-Chyi;Mackel, Julie B.;Merkle, Hellmut;Silva, Afonso C.
通讯作者：
Silva, Afonso C.
影响因子：
5.7
作者：
Liu, Junjie V.;Hirano, Yoshiyuki;Nascimento, George C.;Stefanovic, Bojana;Leopold, David A.;Silva, Afonso C.
通讯作者：
Silva, Afonso C.
影响因子：
5.7
作者：
Pfeuffer, J;McCullough, JC;Hu, XP
通讯作者：
Hu, XP
影响因子：
3.3
作者：
BANDETTINI, PA;JESMANOWICZ, A;HYDE, JS
通讯作者：
HYDE, JS
影响因子：
5.7
作者：
FRISTON, KJ;HOLMES, AP;TURNER, R
通讯作者：
TURNER, R