fMRI in the awake marmoset: somatosensory-evoked responses, functional connectivity, and comparison with propofol anesthesia.
fMRI in the awake marmoset: somatosensory-evoked responses, functional connectivity, and comparison with propofol anesthesia.
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DOI：
10.1016/j.neuroimage.2013.03.038
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发表时间：
2013-09
期刊：
影响因子：
5.7
通讯作者：
Silva, Afonso C.
中科院分区：
文献类型：
--
作者：
Liu, Junjie V.;Hirano, Yoshiyuki;Nascimento, George C.;Stefanovic, Bojana;Leopold, David A.;Silva, Afonso C.
关键词：
动物模型中的功能性神经成像对于理解神经血管耦合的原理和fMRI信号的生理基础是必不可少的，fMRI信号被广泛用于研究人类大脑中的感觉和认知过程。虽然对感觉刺激的血流动力学反应已在人类中表征，但动物研究能够将非常高分辨率的成像与侵入性测量和药理学操作结合联合收割机。迄今为止，大多数高分辨率的研究神经血管耦合在小动物已进行了麻醉啮齿动物。在这里，我们报告的功能磁共振成像实验中有意识的，清醒的普通绒猴（Callithrix jacchus），并比较异丙酚麻醉动物的反应。在有意识的绒猴，强大的BOLD功能磁共振成像反应的体感刺激前臂被发现在对侧和同侧区域的丘脑，初级（SI）和次级（SII）体感皮层，和尾状核。这些反应明显强于麻醉绒猴，并表现出单调增加的幅度BOLD响应与刺激频率。另一方面，麻醉显著减弱丘脑，SI和SII的反应，并取消了尾状核和同侧SI的反应。此外，麻醉影响其他几个方面的功能磁共振成像反应，包括形状的血流动力学反应函数和interreal（SI-SII）自发功能连接。总之，这些发现证明了有意识的，清醒的绒猴模型的价值，在没有麻醉的情况下，在躯体感觉通路的生理反应的研究，使数据可以最直接地比较有意识的人的fMRI。
Functional neuroimaging in animal models is essential for understanding the principles of neurovascular coupling and the physiological basis of fMRI signals that are widely used to study sensory and cognitive processing in the human brain. While hemodynamic responses to sensory stimuli have been characterized in humans, animal studies are able to combine very high resolution imaging with invasive measurements and pharmacological manipulation. To date, most high-resolution studies of neurovascular coupling in small animals have been carried out in anesthetized rodents. Here we report fMRI experiments in conscious, awake common marmosets (Callithrix jacchus), and compare responses to animals anesthetized with propofol. In conscious marmosets, robust BOLD fMRI responses to somatosensory stimulation of the forearm were found in contralateral and ipsilateral regions of the thalamus, primary (SI) and secondary (SII) somatosensory cortex, and the caudate nucleus. These responses were markedly stronger than those in anesthetized marmosets and showed a monotonic increase in the amplitude of the BOLD response with stimulus frequency. On the other hand, anesthesia significantly attenuated responses in thalamus, SI and SII, and abolished responses in caudate and ipsilateral SI. Moreover, anesthesia influenced several other aspects of the fMRI responses, including the shape of the hemodynamic response function and the interareal (SI–SII) spontaneous functional connectivity. Together, these findings demonstrate the value of the conscious, awake marmoset model for studying physiological responses in the somatosensory pathway, in the absence of anesthesia, so that the data can be compared most directly to fMRI in conscious humans.
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影响因子：
3
作者：
Bock, Nicholas A.;Kocharyan, Ara;Liu, Junjie V.;Silva, Afonso C.
通讯作者：
Silva, Afonso C.
影响因子：
4.8
作者：
Gamlin, PD;Ward, MK;Twieg, DB
通讯作者：
Twieg, DB
DOI：
10.1002/ar.22451
发表时间：
2012-05
影响因子：
2
作者：
de la Mothe, Lisa A.;Blumell, Suzanne;Kajikawa, Yoshinao;Hackett, Troy A.
通讯作者：
Hackett, Troy A.
功能磁共振成像对超短刺激的响应的时空演化。
DOI：
10.1523/jneurosci.3986-10.2011
发表时间：
2011-01-26
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hirano Y;Stefanovic B;Silva AC
通讯作者：
Silva AC
影响因子：
2.5
作者：
de la Mothe, LA;Blumell, S;Hackett, TA
通讯作者：
Hackett, TA