Identification of miRNA signatures during the differentiation of hESCs into retinal pigment epithelial cells.
Identification of miRNA signatures during the differentiation of hESCs into retinal pigment epithelial cells.
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DOI：
10.1371/journal.pone.0037224
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fan G
中科院分区：
文献类型：
--
作者：
Hu G;Huang K;Yu J;Gopalakrishna-Pillai S;Kong J;Xu H;Liu Z;Zhang K;Xu J;Luo Y;Li S;Sun YE;Iverson LE;Xue Z;Fan G
视网膜色素上皮（RPE）细胞可以通过胚胎干细胞（ESC）和诱导多能干细胞（iPSC）的体外分化获得。我们之前已经通过对人ESC和iPSC衍生的RPE以及正常胎儿RPE的转录组分析鉴定了87个与RPE细胞分化和功能相关的特征基因。在这里，我们通过小RNA-seq分析了人类ESC及其RPE衍生物中的miRNA表达。与我们之前的转录组分析得出的结论非常相似，我们发现RPE中的总体miRNA景观与ESC和其他分化的体细胞组织不同。我们还在RPE分化的中间阶段分析了miRNA的表达，并鉴定了逐渐上调或下调的miRNA的独特子集，表明这些miRNA的动态调节与RPE分化过程相关。事实上，在RPE分化过程中miRNA亚组的下调与RPE特异性基因的上调相关，例如RPE 65，其仅在RPE中表达。我们的结论是，miRNA的签名可以用来分类不同程度的体外分化的人多能干细胞的RPE。我们认为，RPE特异性的miRNA可能有助于体外RPE的功能成熟，类似于RPE特异性mRNA表达的调节。
Retinal pigment epithelium (RPE) cells can be obtained through in vitro differentiation of both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We have previously identified 87 signature genes relevant to RPE cell differentiation and function through transcriptome analysis of both human ESC- and iPSC-derived RPE as well as normal fetal RPE. Here, we profile miRNA expression through small RNA-seq in human ESCs and their RPE derivatives. Much like conclusions drawn from our previous transcriptome analysis, we find that the overall miRNA landscape in RPE is distinct from ESCs and other differentiated somatic tissues. We also profile miRNA expression during intermediate stages of RPE differentiation and identified unique subsets of miRNAs that are gradually up- or down-regulated, suggesting that dynamic regulation of these miRNAs is associated with the RPE differentiation process. Indeed, the down-regulation of a subset of miRNAs during RPE differentiation is associated with up-regulation of RPE-specific genes, such as RPE65, which is exclusively expressed in RPE. We conclude that miRNA signatures can be used to classify different degrees of in vitro differentiation of RPE from human pluripotent stem cells. We suggest that RPE-specific miRNAs likely contribute to the functional maturation of RPE in vitro, similar to the regulation of RPE-specific mRNA expression.
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影响因子：
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作者：
Langmead, Ben
通讯作者：
Langmead, Ben
DOI：
10.1089/clo.2006.8.189
发表时间：
2006-09-01
期刊：
CLONING AND STEM CELLS
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2011.10.074
发表时间：
2011-11-18
影响因子：
3.1
作者：
Chung, Eun Jee;Chun, Ji Na;Lee, Joon H.
通讯作者：
Lee, Joon H.