Regulation of sexual development of Plasmodium by translational repression.
Regulation of sexual development of Plasmodium by translational repression.
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DOI：
10.1126/science.1125129
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发表时间：
2006-08-04
期刊：
影响因子：
56.9
通讯作者：
Waters, Andrew P.
中科院分区：
文献类型：
--
作者：
Mair, Gunnar R.;Braks, Joanna A. M.;Garver, Lindsey S.;Wiegant, Loop C. A. G.;Hall, Neil;Dirks, Roeland W.;Khan, Shahid M.;Dimopoulos, George;Janse, Chris J.;Waters, Andrew P.
mRNA的翻译抑制在多细胞真核生物的性分化和配子发生中起着重要作用。我们在这里表明，TR和mRNA营业额的关键影响阶段特异性基因表达的原生动物疟原虫。DDX 6类RNA解旋酶，DOZI（受精卵发育受抑制），发现于雌性血液期配子母细胞的细胞质体中，与已知被抑制的mRNA种类形成复合物。pbdozi的遗传破坏抑制了抑制性静止mRNP的形成，并将这些和其他（>350种不同的）转录物靶向降解途径，而不是将它们引导至翻译多核糖体，从而在蚊子减数分裂之前阻止受精卵发育。因此，TR在疟原虫发育中是必不可少的。与DOZI（第一个描述的疟疾寄生虫TR效应蛋白）相关的蛋白质和过程的完整目录可能会导致预防寄生虫发展的新方法。
Translational repression (TR) of mRNAs plays an important role in sexual differentiation and gametogenesis in multicellular eukaryotes. We show here that TR and mRNA turnover are key influences on stage specific gene expression in the protozoan Plasmodium. The DDX6 class RNA helicase, DOZI (development of zygote inhibited), is found in cytoplasmic bodies of female, blood stage gametocytes in a complex with mRNA species known to be translationally repressed. Genetic disruption of pbdozi inhibits the formation of translationally quiescent mRNPs and targets these and other (>350 different) transcripts to the degradation pathway rather than directing them to translating polysomes, preventing zygote development prior to meiosis in the mosquito. Thus TR is essential in malaria parasite development. A full catalogue of the proteins and processes associated with DOZI (the first described malaria parasite TR-effector protein) might lead to novel approaches to prevent parasite development.
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