Tumor necrosis factor alpha: activity dependent expression and promotion of cortical column sleep in rats.
Tumor necrosis factor alpha: activity dependent expression and promotion of cortical column sleep in rats.
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DOI：
10.1016/j.neuroscience.2008.06.066
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发表时间：
2008-09-22
期刊：
影响因子：
3.3
通讯作者：
Krueger JM
中科院分区：
文献类型：
--
作者：
Churchill L;Rector DM;Yasuda K;Fix C;Rojas MJ;Yasuda T;Krueger JM
睡眠期间皮质表面诱发电位 (SEP) 较大，表征皮质柱中类似睡眠的状态。由于肿瘤坏死因子 α (TNF) 可能参与睡眠调节，并且是由清醒活动产生的，因此我们测试了以下假设：直接将 TNF 应用于皮质将在皮质柱内诱导类睡眠状态并增强 SEP 振幅。我们发现，相对于对照热灭活 TNF 显微注射，将 TNF 显微注射到体感皮层表面增强了胡须刺激诱导的 SEP 振幅。我们还确定了胡须刺激是否增强了内源性 TNF 表达。单须双边偏转 2 小时后，可观察到 TNF 免疫反应性 (IR)。激活的体感桶柱的 II-IV 层中 TNF-IR 细胞的数量增加。在两项独立的研究中，多个晶须的单侧偏转 2 小时增加了柱中 II-V 层中 TNF-IR 细胞的数量，这些柱也表现出增强的 Fos-IR。 TNF-IR 还与 NeuN-IR 共定位，表明 TNF 表达在神经元中。总的来说，这些数据与以下假设一致：TNF 是响应神经活动而产生的，反过来又增加了局部类睡眠状态的可能性，这由 SEP 幅度的增加决定。
Cortical surface evoked potentials (SEPs) are larger during sleep and characterize a sleep-like state in cortical columns. Since tumor necrosis factor alpha (TNF) may be involved in sleep regulation and is produced as a consequence of waking activity, we tested the hypothesis that direct application of TNF to the cortex will induce a sleep-like state within cortical columns and enhance SEP amplitudes. We found that microinjection of TNF onto the surface of the somatosensory cortex enhanced whisker stimulation-induced SEP amplitude relative to a control heat-inactivated TNF microinjection. We also determined if whisker stimulation enhanced endogenous TNF expression. TNF immunoreactivity (IR) was visualized after 2 h of bilateral deflection of a single whisker bilaterally. The number of TNF-IR cells increased in layers II–IV of the activated somatosensory barrel column. In two separate studies, unilateral deflection of multiple whiskers for 2 h increased the number of TNF-IR cells in layers II–V in columns that also exhibited enhanced Fos-IR. TNF-IR also colocalized with NeuN-IR suggesting that TNF expression was in neurons. Collectively these data are consistent with the hypotheses that TNF is produced in response to neural activity and in turn enhances the probability of a local sleep-like state as determined by increases in SEP amplitudes.
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影响因子：
2.9
作者：
Churchill, L;Yasuda, K;Kmeger, JM
通讯作者：
Kmeger, JM
影响因子：
2.8
作者：
Krueger, James M.;Rector, David M.;Churchill, Lynn
通讯作者：
Churchill, Lynn
影响因子：
2.9
作者：
De, A;Krueger, JM;Simasko, SA
通讯作者：
Simasko, SA
DOI：
10.1016/0013-4694(85)91035-1
发表时间：
1985-01-01
期刊：
ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY
影响因子：
--
作者：
KNIGHT, RT;BRAILOWSKY, S;SIMPSON, GV
通讯作者：
SIMPSON, GV
影响因子：
2.9
作者：
Rector, DM;Topchiy, IA;Rojas, MJ
通讯作者：
Rojas, MJ