Topographical variations in the polarization sensitivity of articular cartilage as determined by polarization-sensitive optical coherence tomography and polarized light microscopy.

Topographical variations in the polarization sensitivity of articular cartilage as determined by polarization-sensitive optical coherence tomography and polarized light microscopy.
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通过偏振敏感光学相干断层扫描和偏振光显微镜测定关节软骨偏振敏感性的地形变化。

DOI:
10.1117/1.2976422
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发表时间:
2008
影响因子:
3.5
通讯作者:
Peavy,GeorgeM
Peavy,GeorgeM
中科院分区:
医学3区
文献类型:
--
作者:
Xie,Tuqiang;Xia,Yang;Guo,Shuguang;Hoover,Patrick;Chen,Zhongping;Peavy,GeorgeM

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为了了解关节软骨胶原纤维取向的地形变化对偏振敏感光学相干断层扫描(PS-OCT)光学相位图像的影响,我们使用偏振光显微镜(PLM)来量化在PS-OCT成像的相同位置处软骨中胶原结构的取向和相位延迟。OCT实验表明,在完整的牛胫骨平台上,关节软骨在不同位置处的偏振灵敏度具有正常变化。关节软骨在关节面的内侧边缘和中心区域沿垂直轴不沿着极化敏感,但在胫骨的外侧边缘变得极化敏感。如PS-OCT所示,这种光学相位延迟的差异经PLM验证是由胫骨平台不同位置的胶原纤维取向差异引起的。本研究表明,关节内关节软骨胶原结构的正常地形变化对PS-OCT成像检测到的光学相位延迟具有深远的影响,因此在PS-OCT成像可用于评价单个关节表面的健康状况之前,必须了解并绘制特定关节的地形变化。
To understand the influence of topographical variations in collagen fibril orientation of articular cartilage on optical phase images of polarization-sensitive optical coherence tomography (PS-OCT), we use polarized light microscopy (PLM) to quantify the orientation and phase retardation of the collagen architecture in cartilage at the same locations imaged by PS-OCT. The PS-OCT experiments demonstrate that articular cartilage has normal variations in polarization sensitivity at different locations over an intact bovine tibial plateau. Articular cartilage is not polarization sensitive along the vertical axis on the medial edge and central areas of the joint surface, but becomes polarization sensitive on the lateral edge of the tibia. This difference in optical phase retardation, as demonstrated by PS-OCT, is verified by PLM to be caused by differences in collagen fibril orientation at different locations of the tibial plateau. This study demonstrates that normal topographical variations in the collagen architecture of articular cartilage within a joint have a profound influence on the optical phase retardation detected by PS-OCT imaging, and therefore must be understood and mapped for specific joints before PS-OCT imaging can be used for the evaluation of the health status of individual joint surfaces.
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