Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells.
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells.
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DOI：
10.3390/molecules190812827
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发表时间：
2014-08-21
期刊：
影响因子：
--
通讯作者：
Li CY
中科院分区：
文献类型：
--
作者：
Huang WY;Liu YM;Wang J;Wang XN;Li CY
蓝莓果实具有广泛的健康益处，因为它们含有丰富的花青素，这是天然的抗氧化剂。本研究的目的是研究蓝莓的两种主要花色苷（锦葵素-3-葡萄糖苷和锦葵素-3-半乳糖苷）对内皮细胞炎症反应的抑制作用。这两种锦葵素苷能抑制肿瘤坏死因子-α（TNF-α）诱导的单核细胞趋化蛋白-1（MCP-1）、细胞间粘附分子-1（ICAM-1）和血管细胞粘附分子-1（VCAM-1）的表达，并呈浓度依赖性。1 μM的Mv-3-glc可抑制细胞培养上清中MCP-1、ICAM-1和VCAM-1蛋白的表达，抑制率分别为35.9%、54.4%和44.7%，抑制率分别为9.88%和48.6%（p < 0.05）。此外，Mv-3-glc、Mv-3-gal及其混合物在50 μM浓度下对IκBα降解的抑制率分别为84.8%、75.3%和43.2%，p < 0.01），并能阻断p65的核转位，提示其抗炎机制可能是通过核因子-κ B（NF-κB）途径介导的。总的来说，锦葵素-3-葡萄糖苷的抗炎作用优于锦葵素-3-半乳糖苷。这些结果表明，蓝莓是一种很好的抗炎花青素资源，它可以作为一种很有前途的分子，用于开发营养保健品，以防止许多疾病的慢性炎症。
Blueberry fruits have a wide range of health benefits because of their abundant anthocyanins, which are natural antioxidants. The purpose of this study was to investigate the inhibitory effect of blueberry’s two main anthocyanins (malvidin-3-glucoside and malvidin-3-galactoside) on inflammatory response in endothelial cells. These two malvidin glycosides could inhibit tumor necrosis factor-alpha (TNF-α) induced increases of monocyte chemotactic protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) production both in the protein and mRNA levels in a concentration-dependent manner. Mv-3-glc at the concentration of 1 μM could inhibit 35.9% increased MCP-1, 54.4% ICAM-1, and 44.7% VCAM-1 protein in supernatant, as well as 9.88% MCP-1 and 48.6% ICAM-1 mRNA expression (p < 0.05). In addition, they could decrease IκBα degradation (Mv-3-glc, Mv-3-gal, and their mixture at the concentration of 50 μM had the inhibition rate of 84.8%, 75.3%, and 43.2%, respectively, p < 0.01) and block the nuclear translocation of p65, which suggested their anti-inflammation mechanism was mediated by the nuclear factor-kappa B (NF-κB) pathway. In general malvidin-3-glucoside had better anti-inflammatory effect than malvidin-3-galactoside. These results indicated that blueberry is good resource of anti-inflammatory anthocyanins, which can be promising molecules for the development of nutraceuticals to prevent chronic inflammation in many diseases.
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