Verticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence.
Verticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence.
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大丽黄萎病 CFEM 蛋白操纵宿主免疫并对毒力产生不同影响
DOI：
10.1186/s12915-022-01254-x
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发表时间：
2022-02-23
期刊：
影响因子：
5.4
通讯作者：
Chen JY
中科院分区：
文献类型：
--
作者：
Wang D;Zhang DD;Song J;Li JJ;Wang J;Li R;Klosterman SJ;Kong ZQ;Lin FZ;Dai XF;Subbarao KV;Chen JY
黄萎病是一种引起许多重要经济作物维管束枯萎的真菌病原菌。常见的真菌胞外膜(CFEM)结构域蛋白，包括分泌型蛋白，已经与致病相关，但它们在该病原菌中的作用尚不清楚。通过生物信息学分析鉴定了9个富含CFEM结构域的分泌型小半胱氨酸蛋白(VdSCP)，并对它们对宿主免疫反应的不同抑制作用进行了评价。其中两个蛋白，VdSCP76和VdSCP77，由于它们的信号肽而定位于植物质膜上，并介导了对典型效应物诱导的所有免疫反应的广谱抑制。VdSCP76或VdSCP77的缺失显著降低了棉花上大丽弧菌的致病力。此外，VdSCP76和VdSCP77通过在CFEM家族成员中保守的潜在铁结合位点抑制宿主免疫，其特征是7个VdSCP中含有天冬氨酸残基(Asp-type)，而VdSCP76和VdSCP77中存在天冬酰胺残基(Asn-type)。携带Asn型CFEM成员的大丽弧菌对棉花的致病力强于携带Asp型的菌株。在缺铁的木质部中，大丽叶病毒可能利用Asp型CFEM成员螯合铁，利用Asn型CFEM成员抑制免疫，从而在寄主植物中成功定殖和繁殖。网上版载有补充材料，可在10.1186/s12915-022-01254-x查阅。
Verticillium dahliae is a fungal pathogen that causes a vascular wilt on many economically important crops. Common fungal extracellular membrane (CFEM) domain proteins including secreted types have been implicated in virulence, but their roles in this pathogen are still unknown. Nine secreted small cysteine-rich proteins (VdSCPs) with CFEM domains were identified by bioinformatic analyses and their differential suppression of host immune responses were evaluated. Two of these proteins, VdSCP76 and VdSCP77, localized to the plant plasma membrane owing to their signal peptides and mediated broad-spectrum suppression of all immune responses induced by typical effectors. Deletion of either VdSCP76 or VdSCP77 significantly reduced the virulence of V. dahliae on cotton. Furthermore, VdSCP76 and VdSCP77 suppressed host immunity through the potential iron binding site conserved in CFEM family members, characterized by an aspartic acid residue in seven VdSCPs (Asp-type) in contrast with an asparagine residue (Asn-type) in VdSCP76 and VdSCP77. V. dahliae isolates carrying the Asn-type CFEM members were more virulent on cotton than those carrying the Asp-type. In the iron-insufficient xylem, V. dahliae is likely to employ the Asp-type CFEM members to chelate iron, and Asn-type CFEM members to suppress immunity, for successful colonization and propagation in host plants. The online version contains supplementary material available at 10.1186/s12915-022-01254-x.
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