Mitotic transcription and waves of gene reactivation during mitotic exit.
Mitotic transcription and waves of gene reactivation during mitotic exit.
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DOI：
10.1126/science.aal4671
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发表时间：
2017-10-06
期刊：
影响因子：
--
通讯作者：
Zaret KS
中科院分区：
文献类型：
--
作者：
Palozola KC;Donahue G;Liu H;Grant GR;Becker JS;Cote A;Yu H;Raj A;Zaret KS
尽管基因组通常被认为在有丝分裂期间是转录沉默的，但技术限制已经阻止了在有丝分裂和有丝分裂退出期间对转录的敏感作图。因此，间期表达模式被转导到子细胞的方式尚不清楚。我们使用5-乙炔尿苷脉冲标记有丝分裂和有丝分裂退出过程中的转录本，发现许多基因在有丝分裂过程中表现出转录，如FITC-UTP标记、RNA FISH和RT-qPCR所证实的。有丝分裂后的第一轮转录主要激活参与子细胞生长和重建的基因，而不是细胞类型特异性功能。我们建议，细胞的转录模式在很大程度上保留在一个低水平，通过有丝分裂，而在分裂间期观察到的转录幅度重新建立在有丝分裂退出。
Although the genome is generally thought to be transcriptionally silent during mitosis, technical limitations have prevented sensitive mapping of transcription during mitosis and mitotic exit. Thus, the means by which the interphase expression pattern is transduced to daughter cells have been unclear. We used 5-ethynyluridine to pulse-label transcripts during mitosis and mitotic exit and find that many genes exhibit transcription during mitosis, as confirmed by FITC-UTP labeling, RNA FISH, and RT-qPCR. The first round of transcription immediately following mitosis primarily activates genes involved in the growth and rebuilding of daughter cells, rather than cell type-specific functions. We propose that the cell’s transcription pattern is largely retained at a low level through mitosis, whereas the amplitude of transcription observed in interphase is re-established during mitotic exit.
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影响因子：
3.3
作者：
Prasanth, KV;Sacco-Bubulya, PA;Spector, DL
通讯作者：
Spector, DL
影响因子：
8.8
作者：
Liu Y;Pelham-Webb B;Di Giammartino DC;Li J;Kim D;Kita K;Saiz N;Garg V;Doane A;Giannakakou P;Hadjantonakis AK;Elemento O;Apostolou E
通讯作者：
Apostolou E
DOI：
10.1126/science.1236083
发表时间：
2013-11-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Naumova N;Imakaev M;Fudenberg G;Zhan Y;Lajoie BR;Mirny LA;Dekker J
通讯作者：
Dekker J
影响因子：
64.5
作者：
DERMAN, E;KRAUTER, K;DARNELL, JE
通讯作者：
DARNELL, JE
影响因子：
21.3
作者：
Festuccia, Nicola;Dubois, Agnes;Navarro, Pablo
通讯作者：
Navarro, Pablo