Neuronal cell types, projections, and spatial organization of the central amygdala.
Neuronal cell types, projections, and spatial organization of the central amygdala.
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DOI：
10.1016/j.isci.2022.105497
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发表时间：
2022-12-22
期刊：
影响因子：
5.8
通讯作者：
Cembrowski, Mark S.
中科院分区：
文献类型：
--
作者：
O'Leary, Timothy P.;Kendrick, Rennie M.;Bristow, Brianna N.;Sullivan, Kaitlin E.;Wang, Lihua;Clements, Jody;Lemire, Andrew L.;Cembrowski, Mark S.
中央杏仁核（CEA）已被充分研究，根据特定的细胞类型和电路解释功能和行为。这些工作通常通过经典的抑制性标记基因来定义分子细胞类型;因此，标记基因定义的细胞类型是否详尽地覆盖CEA并且与连接性共变仍未解决。在这里，我们结合单细胞RNA测序，多重荧光原位杂交，免疫组织化学和远程投影映射，以获得一个“自下而上”的CEA细胞类型的理解。在此过程中，我们确定了两种主要的细胞类型，涵盖了所有CEA神经元的三分之一，这些细胞类型在之前的研究中尚未得到解决。在空间映射这些新的类型，我们确定了一个非典型的CEA子域与Nr 2f 2的表达，并揭示了一个Isl 1表达的中膜细胞类型，占许多长距离的CEA预测。我们的研究结果揭示了跨细胞类型和空间尺度的新CEA组织原则，并为未来研究细胞类型特异性行为和功能的工作提供了框架。用scRNA-seq和多重FISH检查中央杏仁核神经元我们的工作揭示了特定空间域中的新抑制性神经元细胞类型新揭示的亚型具有专门的蛋白质和长程投射我们的工作阐明了中央杏仁核的空间细胞类型特异性景观生物科学;神经科学;细胞神经科学;组学;转录组学
The central amygdala (CEA) has been richly studied for interpreting function and behavior according to specific cell types and circuits. Such work has typically defined molecular cell types by classical inhibitory marker genes; consequently, whether marker-gene-defined cell types exhaustively cover the CEA and co-vary with connectivity remains unresolved. Here, we combined single-cell RNA sequencing, multiplexed fluorescent in situ hybridization, immunohistochemistry, and long-range projection mapping to derive a “bottom-up” understanding of CEA cell types. In doing so, we identify two major cell types, encompassing one-third of all CEA neurons, that have gone unresolved in previous studies. In spatially mapping these novel types, we identify a non-canonical CEA subdomain associated with Nr2f2 expression and uncover an Isl1-expressing medial cell type that accounts for many long-range CEA projections. Our results reveal new CEA organizational principles across cell types and spatial scales and provide a framework for future work examining cell-type-specific behavior and function. Central amygdala neurons were examined with scRNA-seq and multiplexed FISH Our work revealed new inhibitory neuron cell types within specific spatial domains Newly revealed subtypes had specialized proteins and long-range projections Our work clarifies the spatial cell-type-specific landscape of the central amygdala Biological sciences; Neuroscience; Cellular neuroscience; Omics; Transcriptomics
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影响因子：
7.7
作者：
Cembrowski MS;Wang L;Lemire AL;Copeland M;DiLisio SF;Clements J;Spruston N
通讯作者：
Spruston N
影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
64.8
作者：
Ciocchi, Stephane;Herry, Cyril;Luethi, Andreas
通讯作者：
Luethi, Andreas
DOI：
10.1016/b978-0-12-815134-1.00003-9
发表时间：
2020-01-01
期刊：
Handbook of behavioral neuroscience
影响因子：
--
作者：
Beyeler, Anna;Dabrowska, Joanna
通讯作者：
Dabrowska, Joanna