Pathway-specific utilization of synaptic zinc in the macaque ventral visual cortical areas.
Pathway-specific utilization of synaptic zinc in the macaque ventral visual cortical areas.
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DOI：
10.1093/cercor/bhq028
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Rockland KS
中科院分区：
文献类型：
--
作者：
Ichinohe N;Matsushita A;Ohta K;Rockland KS
突触锌是一种活动相关的神经调质，富集在海马苔藓纤维和一个子集的突触能皮质投射，不包括丘脑皮质或皮质丘脑。在啮齿类动物中已经报道了突触锌利用的一定程度的途径特异性。在这里，我们使用局灶性注射的逆行示踪剂亚硒酸钠，以确定锌阳性（Zn+）投射神经元在猴子腹侧视觉通路。在V1，V4和TEO区域注射后，在几个反馈通路中优先检测到神经元，但不寻常的是，仅限于更深层，而不涉及第2层或第3层。颞注射导致更广泛的标签反馈和颞内关联途径。锌+神经元有一个更广泛的层状分布，类似于标准的逆行示踪剂的结果。在TE后区顺行注射示踪剂后，电镜分析证实了一部分反馈突触被锌标记。附近的注射，Zn+内在神经元集中在第2层，但在颞区也丰富的第6层。这些结果表明相当大的途径和层状特异性，皮质神经元使用突触锌。鉴于突触锌的假设作用，这可能导致不同的突触特性，可能包括不同的突触可塑性内或跨投影。
Synaptic zinc is an activity-related neuromodulator, enriched in hippocampal mossy fibers and a subset of glutamatergic cortical projections, exclusive of thalamocortical or corticothalamic. Some degree of pathway specificity in the utilization of synaptic zinc has been reported in rodents. Here, we use focal injections of the retrograde tracer sodium selenite to identify zinc-positive (Zn+) projection neurons in the monkey ventral visual pathway. After injections in V1, V4, and TEO areas, neurons were detected preferentially in several feedback pathways but, unusually, were restricted to deeper layers without involvement of layers 2 or 3. Temporal injections resulted in more extensive labeling of both feedback and intratemporal association pathways. The Zn+ neurons had a broader laminar distribution, similar to results from standard retrograde tracers. After anterograde tracer injection in area posterior TE, electron microscopic analysis substantiated that a proportion of feedback synapses was colabeled with zinc. Nearby injections, Zn+ intrinsic neurons concentrated in layer 2, but in temporal areas were also abundant in layer 6. These results indicate considerable pathway and laminar specificity as to which cortical neurons use synaptic zinc. Given the hypothesized roles of synaptic zinc, this is likely to result in distinct synaptic properties, possibly including differential synaptic plasticity within or across projections.
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