Retrotransposon-based molecular markers for analysis of genetic diversity within the Genus Linum.

Retrotransposon-based molecular markers for analysis of genetic diversity within the Genus Linum.
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DOI:
10.1155/2014/231589
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发表时间:
2014
影响因子:
--
通讯作者:
Muravenko OV
Muravenko OV
中科院分区:
生物学3区
文献类型:
--
作者:
Melnikova NV;Kudryavtseva AV;Zelenin AV;Lakunina VA;Yurkevich OY;Speranskaya AS;Dmitriev AA;Krinitsina AA;Belenikin MS;Uroshlev LA;Snezhkina AV;Sadritdinova AF;Koroban NV;Amosova AV;Samatadze TE;Guzenko EV;Lemesh VA;Savilova AM;Rachinskaia OA;Kishlyan NV;Rozhmina TA;Bolsheva NL;Muravenko OV

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采用SSAP方法对亚麻属亚麻组(Linum)、腺叶亚麻组(Adenolinum)、毛叶亚麻组(Dasylinum)、繁缕亚麻组(Stellerolinum)的22个种和46个亚麻栽培品种的遗传多样性进行了研究。所有研究的亚麻品种区分使用SSAP反转录转座子FL9和FL11。因此,SSAP方法的有效性证明了亚麻标记,在基因库收集的种质鉴定,并在亚麻品种繁殖过程中控制。Fl1a、Fl1b和Cassandra插入片段在亚麻品种中的多态性很低,但这些反转录转座子已成功地用于亚麻属物种的调查。基于SSAP标记的物种聚类与其分类学划分为Dasylinum,Stellerolinum,Adenolinum和Linum的一致。Adenolinum组的所有种都与亚麻组的种分开聚簇。数据证实了这些部分中分离的准确性。亚麻组的成员不像其他组的成员那样密切相关,因此本组的分类修订是可取的。L.在我们的工作中揭示了与现代亚麻栽培品种遗传上相距甚远的usitatissimum种质。这些材料对于亚麻育种和将新的有用性状引入亚麻品种具有极其重要的意义。对Cassandra反转录转座子在亚麻属植物中的染色体定位进行了研究。
SSAP method was used to study the genetic diversity of 22 Linum species from sections Linum, Adenolinum, Dasylinum, Stellerolinum, and 46 flax cultivars. All the studied flax varieties were distinguished using SSAP for retrotransposons FL9 and FL11. Thus, the validity of SSAP method was demonstrated for flax marking, identification of accessions in genebank collections, and control during propagation of flax varieties. Polymorphism of Fl1a, Fl1b, and Cassandra insertions were very low in flax varieties, but these retrotransposons were successfully used for the investigation of Linum species. Species clusterization based on SSAP markers was in concordance with their taxonomic division into sections Dasylinum, Stellerolinum, Adenolinum, and Linum. All species of sect. Adenolinum clustered apart from species of sect. Linum. The data confirmed the accuracy of the separation in these sections. Members of section Linum are not as closely related as members of other sections, so taxonomic revision of this section is desirable. L. usitatissimum accessions genetically distant from modern flax cultivars were revealed in our work. These accessions are of utmost interest for flax breeding and introduction of new useful traits into flax cultivars. The chromosome localization of Cassandra retrotransposon in Linum species was determined.
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