Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract.
Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract.
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DOI：
10.1038/s41598-017-19079-y
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发表时间：
2018-01-16
影响因子：
4.6
通讯作者：
Davies RL
中科院分区：
文献类型：
--
作者：
Cozens D;Grahame E;Sutherland E;Taylor G;Berry CC;Davies RL
牛易受各种细菌和病毒病原体的经济上重要的呼吸道感染，迫切需要开发更真实的牛呼吸道体外模型，以提高我们对疾病发病机制的认识。在本研究中，我们优化了无血清培养基中的培养条件，使牛支气管上皮细胞（BBECs）生长在空气-液体界面分化成三维上皮，这是高度代表性的牛气道。表皮生长因子是触发BBEC增殖和分化所必需的，而维甲酸也是粘液纤毛分化所必需的。三碘甲腺原氨酸被证明是不重要的分化的BBEC。氧浓度有一个最小的影响，虽然最佳的纤毛时，BBEC培养在14%的氧张力。插入孔密度对BBEC的生长和分化有显着的影响;高孔密度是触发最佳分化所必需的。已建立的BBEC模型将广泛应用于牛呼吸道细菌和病毒感染的研究;它将有助于改进疫苗和治疗方法的开发，并将减少牛在体内实验中的使用。
Cattle are subject to economically-important respiratory tract infections by various bacterial and viral pathogens and there is an urgent need for the development of more realistic in vitro models of the bovine respiratory tract to improve our knowledge of disease pathogenesis. In the present study, we have optimized the culture conditions in serum-free medium that allow bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface to differentiate into a three-dimensional epithelium that is highly representative of the bovine airway. Epidermal growth factor was required to trigger both proliferation and differentiation of BBECs whilst retinoic acid was also essential for mucociliary differentiation. Triiodothyronine was demonstrated not to be important for the differentiation of BBECs. Oxygen concentration had a minimal effect although optimal ciliation was achieved when BBECs were cultured at 14% oxygen tension. Insert pore-density had a significant effect on the growth and differentiation of BBECs; a high-pore-density was required to trigger optimum differentiation. The established BBEC model will have wide-ranging applications for the study of bacterial and viral infections of the bovine respiratory tract; it will contribute to the development of improved vaccines and therapeutics and will reduce the use of cattle in in vivo experimentation.
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DOI：
10.1016/j.jcf.2004.05.010
发表时间：
2004-08-01
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
--
作者：
Bals, Robert;Beisswenger, Christoph;Chinet, Thierry
通讯作者：
Chinet, Thierry
影响因子：
3.7
作者：
Hackett NR;Shaykhiev R;Walters MS;Wang R;Zwick RK;Ferris B;Witover B;Salit J;Crystal RG
通讯作者：
Crystal RG
影响因子：
3.3
作者：
Boukahil, Ismail;Czuprynski, Charles J.
通讯作者：
Czuprynski, Charles J.
影响因子：
3.3
作者：
Blondeau, J. M.;Borsos, S.;Hesje, C. E.
通讯作者：
Hesje, C. E.
影响因子：
5.8
作者：
Griggs TF;Bochkov YA;Basnet S;Pasic TR;Brockman-Schneider RA;Palmenberg AC;Gern JE
通讯作者：
Gern JE