A hierarchical bilayer architecture for complex tissue regeneration.
A hierarchical bilayer architecture for complex tissue regeneration.
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用于复杂组织再生的分层双层结构
DOI：
10.1016/j.bioactmat.2021.08.024
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发表时间：
2022-04
影响因子：
18.9
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Yu M;Luo D;Qiao J;Guo J;He D;Jin S;Tang L;Wang Y;Shi X;Mao J;Cui S;Fu Y;Li Z;Liu D;Zhang T;Zhang C;Li Z;Zhou Y;Liu Y
Engineering a complete, physiologically functional, periodontal complex structure remains a great clinical challenge due to the highly hierarchical architecture of the periodontium and coordinated regulation of multiple growth factors required to induce stem cell multilineage differentiation. Using biomimetic self-assembly and microstamping techniques, we construct a hierarchical bilayer architecture consisting of intrafibrillarly mineralized collagen resembling bone and cementum, and unmineralized parallel-aligned fibrils mimicking periodontal ligament. The prepared biphasic scaffold possesses unique micro/nano structure, differential mechanical properties, and growth factor-rich microenvironment between the two phases, realizing a perfect simulation of natural periodontal hard/soft tissue interface. The interconnected porous hard compartment with a Young's modulus of 1409.00 ± 160.83 MPa could induce cross-arrangement and osteogenic differentiation of stem cells in vitro, whereas the micropatterned soft compartment with a Young's modulus of 42.62 ± 4.58 MPa containing abundant endogenous growth factors, could guide parallel arrangement and fibrogenic differentiation of stem cells in vitro. After implantation in critical-sized complete periodontal tissue defect, the biomimetic bilayer architecture potently reconstructs native periodontium with the insertion of periodontal ligament fibers into newly formed cementum and alveolar bone by recruiting host mesenchymal stem cells and activating the transforming growth factor beta 1/Smad3 signaling pathway. Taken together, integration of self-assembly and microstamping strategies could successfully fabricate a hierarchical bilayer architecture, which exhibits great potential for recruiting and regulating host stem cells to promote synergistic regeneration of hard/soft tissues. •A CGF/IMC biphasic scaffold is made by a self-assembly integrated microstamping strategy. •CGF/IMC mimics periodontium in micro/nano structure, mechanics and growth factor environment. •CGF/IMC bidirectionally regulates osteogenic/fibrogenic differentiation of stem cells. •CGF/IMC achieves complete periodontal regeneration by recruiting host stem cells.
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影响因子：
14
作者：
Park, Chan Ho;Rios, Hector F.;Jin, Qiming;Bland, Megan E.;Flanagan, Colleen L.;Hollister, Scott J.;Giannobile, William V.
通讯作者：
Giannobile, William V.
影响因子：
7.6
作者：
King, GN;King, N;Hughes, FJ
通讯作者：
Hughes, FJ
热力学控制的分层交错结构自组装作为自体骨移植物的骨诱导替代方案（IF：18.808）
影响因子：
19
作者：
Liu, Yan;Luo, Dan;Zhou, Yanheng
通讯作者：
Zhou, Yanheng
影响因子：
7.2
作者：
Kim, Kevin K.;Sheppard, Dean;Chapman, Harold A.
通讯作者：
Chapman, Harold A.
影响因子：
9.5
作者：
Fu, Yu;Liu, Shuai;Zhou, Yan-Heng
通讯作者：
Zhou, Yan-Heng