Solanaceous exocyst subunits are involved in immunity to diverse plant pathogens.
Solanaceous exocyst subunits are involved in immunity to diverse plant pathogens.
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DOI：
10.1093/jxb/erx442
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发表时间：
2018-01-23
影响因子：
6.9
通讯作者：
Bouwmeester K
中科院分区：
文献类型：
--
作者：
Du Y;Overdijk EJR;Berg JA;Govers F;Bouwmeester K
这项研究提供了对茄科植物胞囊亚基进化的深入了解，并首次展示了它们在对抗多种不相关病原体的免疫中所起的作用。胞囊是一种由8个亚基组成的多蛋白复合物，通过介导高尔基后衍生囊泡向质膜的分泌，在许多生物过程中发挥重要作用。近年来，植物囊泡亚基在病原体防御中的作用已经被发现，这主要是基于对模式植物拟南芥的研究。在包括作物在内的其他植物物种中，只有少数研究对胞囊亚基在植物防御中的作用进行了研究。在本研究中，通过挖掘来自茄科植物benthamiana Nicotiana、西红柿和马铃薯的数据库，从胞囊亚基中获得预测的蛋白质序列。随后，分析了它们与拟南芥胞囊亚基的进化关系。benthamiana的基因沉默表明，为了抵御（半）生物营养植物病原体疫霉和丁香假单胞菌，植物需要几个胞囊亚基。相反，一些囊胞亚基似乎是坏死性真菌葡萄球菌的易感因子。此外，大多数胞囊亚基被发现参与胼胝质沉积，表明它们在基础植物防御中起作用。这项研究提供了对茄科植物胞囊亚基进化的深入了解，并首次展示了它们在对抗多种不相关病原体的免疫中所起的作用。
This study provides insight into the evolution of the exocyst subunits of Solanaceous plants and is the first to show their role in immunity against multiple unrelated pathogens. The exocyst, a multiprotein complex consisting of eight subunits, plays an essential role in many biological processes by mediating secretion of post-Golgi-derived vesicles towards the plasma membrane. In recent years, roles for plant exocyst subunits in pathogen defence have been uncovered, largely based on studies in the model plant Arabidopsis. Only a few studies have been undertaken to assign the role of exocyst subunits in plant defence in other plants species, including crops. In this study, predicted protein sequences from exocyst subunits were retrieved by mining databases from the Solanaceous plants Nicotiana benthamiana, tomato, and potato. Subsequently, their evolutionary relationship with Arabidopsis exocyst subunits was analysed. Gene silencing in N. benthamiana showed that several exocyst subunits are required for proper plant defence against the (hemi-)biotrophic plant pathogens Phytophthora infestans and Pseudomonas syringae. In contrast, some exocyst subunits seem to act as susceptibility factors for the necrotrophic pathogen Botrytis cinerea. Furthermore, the majority of the exocyst subunits were found to be involved in callose deposition, suggesting that they play a role in basal plant defence. This study provides insight into the evolution of exocyst subunits in Solanaceous plants and is the first to show their role in immunity against multiple unrelated pathogens.
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影响因子：
9.4
作者：
Chong, Yolanda T.;Gidda, Satinder K.;Goring, Daphne R.
通讯作者：
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影响因子：
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9.4
作者：
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通讯作者：
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DOI：
10.1073/pnas.94.26.14438
发表时间：
1997-12-23
影响因子：
11.1
作者：
Kee, Y;Yoo, JS;Scheller, RH
通讯作者：
Scheller, RH