The genome resources for conservation of Indo-Pacific humpback dolphin, Sousa chinensis
The genome resources for conservation of Indo-Pacific humpback dolphin, Sousa chinensis
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用于保护印度太平洋座头海豚 Sousa chinensis 的基因组资源
DOI：
10.1038/s41597-019-0078-6
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发表时间：
2019-05
期刊：
影响因子：
9.8
通讯作者：
Liu Wenhua
中科院分区：
文献类型：
--
作者：
Ming Yao;Jian Jianbo;Yu Xueying;Wang Jingzhen;Liu Wenhua
中华海豚（Sousa chinensis）是一种受威胁的海洋哺乳动物，属国家一级重点保护野生水生动物名录。然而，有限的基因组信息可用于其种群遗传学和生物保护的研究。在这里，我们已经组装了这个物种的基因组序列使用全基因组鸟枪（WGS）测序策略后，试点低覆盖率的基因组调查。总组装的基因组大小为2.34 Gb：重叠群N50为67 kb，支架N50为9 Mb（107.6倍测序覆盖率）。色葡萄中华绒螯蟹基因组包含24，640个预测的蛋白质编码基因，约37%的重复序列。通过对通用单拷贝直向同源基因（BUSCO）进行基准测试来评估基因组组装的完整性：在总共4，104个预期的哺乳动物基因中，94.3%被鉴定为完整，2.3%被鉴定为片段化。这种高质量的基因组组装和注释技术将极大地促进未来的遗传多样性、保护和进化研究。
The Indo-Pacific humpback dolphin (Sousa chinensis), is a threatened marine mammal and belongs to the First Order of the National Key Protected Wild Aquatic Animals List in China. However, limited genomic information is available for studies of its population genetics and biological conservation. Here, we have assembled a genomic sequence of this species using a whole genome shotgun (WGS) sequencing strategy after a pilot low coverage genome survey. The total assembled genome size was 2.34 Gb: with a contig N50 of 67 kb and a scaffold N50 of 9 Mb (107.6-fold sequencing coverage). The S. chinensis genome contained 24,640 predicted protein-coding genes and had approximately 37% repeated sequences. The completeness of the genome assembly was evaluated by benchmarking universal single copy orthologous genes (BUSCOs): 94.3% of a total 4,104 expected mammalian genes were identified as complete, and 2.3% were identified as fragmented. This newly produced high-quality assembly and annotation of the genome will greatly promote the future studies of the genetic diversity, conservation and evolution.
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影响因子：
9.2
作者：
Buti M;Moretto M;Barghini E;Mascagni F;Natali L;Brilli M;Lomsadze A;Sonego P;Giongo L;Alonge M;Velasco R;Varotto C;Šurbanovski N;Borodovsky M;Ward JA;Engelen K;Cavallini A;Cestaro A;Sargent DJ
通讯作者：
Sargent DJ
影响因子：
--
作者：
Bingyao Chen;Xinrong Xu;T. Jefferson;P. Olson;Qiurong Qin;Hongke Zhang;Liwen He;Guang Yang
通讯作者：
Bingyao Chen;Xinrong Xu;T. Jefferson;P. Olson;Qiurong Qin;Hongke Zhang;Liwen He;Guang Yang
影响因子：
30.8
作者：
Yim HS;Cho YS;Guang X;Kang SG;Jeong JY;Cha SS;Oh HM;Lee JH;Yang EC;Kwon KK;Kim YJ;Kim TW;Kim W;Jeon JH;Kim SJ;Choi DH;Jho S;Kim HM;Ko J;Kim H;Shin YA;Jung HJ;Zheng Y;Wang Z;Chen Y;Chen M;Jiang A;Li E;Zhang S;Hou H;Kim TH;Yu L;Liu S;Ahn K;Cooper J;Park SG;Hong CP;Jin W;Kim HS;Park C;Lee K;Chun S;Morin PA;O'Brien SJ;Lee H;Kimura J;Moon DY;Manica A;Edwards J;Kim BC;Kim S;Wang J;Bhak J;Lee HS;Lee JH
通讯作者：
Lee JH
DOI：
10.1126/science.1252806
发表时间：
2014-06-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jiang Y;Xie M;Chen W;Talbot R;Maddox JF;Faraut T;Wu C;Muzny DM;Li Y;Zhang W;Stanton JA;Brauning R;Barris WC;Hourlier T;Aken BL;Searle SMJ;Adelson DL;Bian C;Cam GR;Chen Y;Cheng S;DeSilva U;Dixen K;Dong Y;Fan G;Franklin IR;Fu S;Guan R;Highland MA;Holder ME;Huang G;Ingham AB;Jhangiani SN;Kalra D;Kovar CL;Lee SL;Liu W;Liu X;Lu C;Lv T;Mathew T;McWilliam S;Menzies M;Pan S;Robelin D;Servin B;Townley D;Wang W;Wei B;White SN;Yang X;Ye C;Yue Y;Zeng P;Zhou Q;Hansen JB;Kristensen K;Gibbs RA;Flicek P;Warkup CC;Jones HE;Oddy VH;Nicholas FW;McEwan JC;Kijas J;Wang J;Worley KC;Archibald AL;Cockett N;Xu X;Wang W;Dalrymple BP
通讯作者：
Dalrymple BP
影响因子：
4.9
作者：
Bao W;Kojima KK;Kohany O
通讯作者：
Kohany O