Mitochondrial genomes of blister beetles (Coleoptera, Meloidae) and two large intergenic spacers in Hycleus genera.
Mitochondrial genomes of blister beetles (Coleoptera, Meloidae) and two large intergenic spacers in Hycleus genera.
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DOI：
10.1186/s12864-017-4102-y
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发表时间：
2017-09-06
期刊：
影响因子：
4.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Du C;Zhang L;Lu T;Ma J;Zeng C;Yue B;Zhang X
昆虫线粒体基因组（线粒体基因组）在某些谱系中表现出高度多样性。尽管人们对Meloidae的有丝分裂基因组知之甚少，但在几种鞘翅目物种中已经报道了基因重排和大基因间隔区（IGS）。我们确定了七个根系物种的完整或接近完整的有丝分裂基因组。环状基因组编码 13 个蛋白质编码基因 (PCG)、22 个转移 RNA (tRNA) 和两个核糖体 RNA (rRNA)，并包含一个控制区域，其基因排列与昆虫的祖先类型相同。所有 PCG 的进化速率表明它们的进化基于纯化选择。 tRNA 二级结构的比较表明了 Meloidae 中不同的取代模式。值得注意的是，所研究的三个 Hycleus 物种的所有有丝分裂基因组都包含两个大的基因间间隔区 (IGS)。 IGS1 位于 trnW 和 trnC 之间，包含 9 bp 共有基序。 IGS2 位于 trnS2 (UCN) 和 nad1 之间，包含不连续的五核苷酸基序重复序列和两端的两个 18 bp 重复单元。迄今为止，在所有已发表的鞘翅目有丝分裂基因组中，IGS2 仅在 Hycleus 属中发现。重复/随机丢失模型和滑链错配被提议作为两个 IGS（IGS1、IGS2）的进化机制。使用 MrBayes、RAxML 和 PhyloBayes 方法基于所有已发表的拟步甲类线粒体基因组中 13 个 PCG 的核苷酸和氨基酸数据集进行系统发育分析，一致地恢复了拟甲虫科和拟甲虫科的单系性。在Meloidae中，Lytta属与Epicauta聚集在一起，而不是与Mylabris聚集在一起。尽管迄今为止收集的数据无法解决Meloidae内的系统发育关系，但这项研究将有助于未来Meloidae系统发育的绘制。这项研究提出了七种根甲虫的有丝分裂基因组。新的线粒体基因组保留了鞘翅目祖先的基因组结构，但在所研究的三个 Hycleus 物种中包含两个 IGS。两种 IGS 的比较分析表明，它们的进化机制是重复/随机丢失模型和滑链错配。本文的在线版本 (10.1186/s12864-017-4102-y) 包含补充材料，可供授权用户使用。
Insect mitochondrial genomes (mitogenomes) exhibit high diversity in some lineages. The gene rearrangement and large intergenic spacer (IGS) have been reported in several Coleopteran species, although very little is known about mitogenomes of Meloidae. We determined complete or nearly complete mitogenomes of seven meloid species. The circular genomes encode 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs) and two ribosomal RNAs (rRNAs), and contain a control region, with gene arrangement identical to the ancestral type for insects. The evolutionary rates of all PCGs indicate that their evolution is based on purifying selection. The comparison of tRNA secondary structures indicates diverse substitution patterns in Meloidae. Remarkably, all mitogenomes of the three studied Hycleus species contain two large intergenic spacers (IGSs). IGS1 is located between trnW and trnC, including a 9 bp consensus motif. IGS2 is located between trnS2 (UCN) and nad1, containing discontinuous repeats of a pentanucleotide motif and two 18-bp repeat units in both ends. To date, IGS2 is found only in genera Hycleus across all published Coleopteran mitogenomes. The duplication/random loss model and slipped-strand mispairing are proposed as evolutionary mechanisms for the two IGSs (IGS1, IGS2). The phylogenetic analyses using MrBayes, RAxML, and PhyloBayes methods based on nucleotide and amino acid datasets of 13 PCGs from all published mitogenomes of Tenebrionoids, consistently recover the monophylies of Meloidae and Tenebrionidae. Within Meloidae, the genus Lytta clusters with Epicauta rather than with Mylabris. Although data collected thus far could not resolve the phylogenetic relationships within Meloidae, this study will assist in future mapping of the Meloidae phylogeny. This study presents mitogenomes of seven meloid beetles. New mitogenomes retain the genomic architecture of the Coleopteran ancestor, but contain two IGSs in the three studied Hycleus species. Comparative analyses of two IGSs suggest that their evolutionary mechanisms are duplication/random loss model and slipped-strand mispairing. The online version of this article (10.1186/s12864-017-4102-y) contains supplementary material, which is available to authorized users.
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