Detection of four human coronaviruses in respiratory infections in children: a one-year study in Colorado.
Detection of four human coronaviruses in respiratory infections in children: a one-year study in Colorado.
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儿童呼吸道感染中四种人类冠状病毒的检测：科罗拉多州的一项为期一年的研究。
DOI：
10.1002/jmv.21541
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发表时间：
2009-09
影响因子：
12.7
通讯作者：
Holmes, Kathryn V.
中科院分区：
文献类型：
--
作者：
Dominguez, Samuel R.;Robinson, Christine C.;Holmes, Kathryn V.
下呼吸道感染是全世界儿童死亡的主要原因。需要采用敏感的聚合酶链反应（PCR）方法对四种人类非SARS人冠状病毒（HCoV）的流行病学和临床相关性进行研究，以评估全球HCoV感染的临床意义。在1年期间（2004年12月- 2005年11月）提交给诊断病毒学实验室的儿童呼吸道标本（1683份）采用常规方法检测7种呼吸道病毒呈阴性，使用灵敏的RT - PCR方法检测4种hcov的RNA。84例（5.0%）标本中检测到冠状病毒rna: HCoV‐NL63 37例，HCoV‐OC43 34例，HCoV‐229E 11例，HCoV‐HKU1 2例。大多数HCoV感染发生在冬季月份，62%以上为以前健康的儿童。26例（41%）冠状病毒阳性患者出现下呼吸道感染（LRTI）， 17例（26%）出现呕吐和/或腹泻，5例（8%）出现脑膜脑炎或癫痫发作。一名免疫功能低下患者的呼吸道标本HCoV - 229E RNA持续阳性3个月。HCoV‐NL63‐阳性患者住院（P = 0.02）和LRTI （P = 0.04）的可能性几乎是HCoV‐OC43‐阳性患者的两倍。在科罗拉多州，hcov与儿童上呼吸道和下呼吸道疾病相关，数量虽少，但意义重大（至少占提交样本总数的2.4%）。我们的数据提出了HCoV可能在胃肠道和中枢神经系统疾病中发挥作用的可能性。需要进一步的研究来调查hcov在这些疾病中的潜在作用。中华医学杂志，2009,31(1):387 - 398。©2009 Wiley‐Liss, Inc。
Lower respiratory tract infections are the leading cause of death in children worldwide. Studies on the epidemiology and clinical associations of the four human non‐SARS human coronaviruses (HCoVs) using sensitive polymerase chain reaction (PCR) assays are needed to evaluate the clinical significance of HCoV infections worldwide. Pediatric respiratory specimens (1,683) submitted to a diagnostic virology laboratory over a 1‐year period (December 2004–November 2005) that were negative for seven respiratory viruses by conventional methods were tested for RNA of four HCoVs using sensitive RT‐PCR assays. Coronavirus RNAs were detected in 84 (5.0%) specimens: HCoV‐NL63 in 37 specimens, HCoV‐OC43 in 34, HCoV‐229E in 11, and HCoV‐HKU1 in 2. The majority of HCoV infections occurred during winter months, and over 62% were in previously healthy children. Twenty‐six (41%) coronavirus positive patients had evidence of a lower respiratory tract infection (LRTI), 17 (26%) presented with vomiting and/or diarrhea, and 5 (8%) presented with meningoencephalitis or seizures. Respiratory specimens from one immunocompromised patient were persistently positive for HCoV‐229E RNA for 3 months. HCoV‐NL63‐positive patients were nearly twice as likely to be hospitalized (P = 0.02) and to have a LRTI (P = 0.04) than HCoV‐OC43‐positive patients. HCoVs are associated with a small, but significant number (at least 2.4% of total samples submitted), of both upper and lower respiratory tract illnesses in children in Colorado. Our data raise the possibility that HCoV may play a role in gastrointestinal and CNS disease. Additional studies are needed to investigate the potential roles of HCoVs in these diseases. J. Med. Virol. 81:1597–1604, 2009. © 2009 Wiley‐Liss, Inc.
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影响因子：
3.1
作者：
Bellau-Pujol S;Vabret A;Legrand L;Dina J;Gouarin S;Petitjean-Lecherbonnier J;Pozzetto B;Ginevra C;Freymuth F
通讯作者：
Freymuth F
影响因子：
11.8
作者：
Chiu, SS;Chan, KH;Peiris, JSM
通讯作者：
Peiris, JSM
影响因子：
12.7
作者：
Coiras, MT;Aguilar, JC;Pérez-Breña, P
通讯作者：
Pérez-Breña, P
影响因子：
12.7
作者：
Ebihara, T;Endo, R;Kikuta, H
通讯作者：
Kikuta, H
影响因子：
11.8
作者：
Briese T;Renwick N;Venter M;Jarman RG;Ghosh D;Köndgen S;Shrestha SK;Hoegh AM;Casas I;Adjogoua EV;Akoua-Koffi C;Myint KS;Williams DT;Chidlow G;van den Berg R;Calvo C;Koch O;Palacios G;Kapoor V;Villari J;Dominguez SR;Holmes KV;Harnett G;Smith D;Mackenzie JS;Ellerbrok H;Schweiger B;Schønning K;Chadha MS;Leendertz FH;Mishra AC;Gibbons RV;Holmes EC;Lipkin WI
通讯作者：
Lipkin WI