Antigen-specific T cell phenotyping microarrays using grating coupled surface plasmon resonance imaging and surface plasmon coupled emission.
Antigen-specific T cell phenotyping microarrays using grating coupled surface plasmon resonance imaging and surface plasmon coupled emission.
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DOI：
10.1016/j.bios.2011.10.029
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发表时间：
2012-01-15
影响因子：
12.6
通讯作者：
Lynes, Michael A.
中科院分区：
文献类型：
--
作者：
Rice, James M.;Stern, Lawrence J.;Guignon, Ernest F.;Lawrence, David A.;Lynes, Michael A.
从血液样本中获得的周围T淋巴细胞的循环种群可以提供有关个人医疗状况和病史的大量信息。最近的证据表明，循环人群中抗原特异性T细胞子集的检测和功能表征可能提供疾病的诊断指标，并有可能预测个人对治疗的反应。在本报告中，结合了光栅耦合表面等离子体共振成像（GCSPRI）和光栅耦合表面等离子体偶联发射（SPCE）荧光检测方式的微阵列检测平台，用于检测和表征CD4+ T细胞。阵列上印刷的感兴趣的微孔区（ROI）由固定的抗体或肽载荷的MHC单体（P/MHC）组成，因为T细胞捕获配体与其他抗体混合在一起，因为细胞因子捕获配体共同结合与腐蚀金传感器碎屑的表面共同结合。使用优化的参数，可以使用GCSPRI在混合T细胞样品中以0.1％的频率检测到未标记的流感肽反应性T细胞克隆。此外，在定量细胞结合后，使用基于SPCE荧光的测定法检测到在Th1或Th2诱导条件下培养的T细胞克隆的差异Th1细胞因子分泌模式。 3种细胞因子的分泌模式的差异（诱导条件的特征）表明差异是捕获细胞功能状态的结果。双模式GCSPRI/SPCE分析可以提供快速，高含量的T细胞筛选/表征工具，可用于诊断疾病，评估疫苗接种功效或评估对免疫治疗药的反应。
The circulating population of peripheral T lymphocytes obtained from a blood sample can provide a large amount of information about an individual's medical status and history. Recent evidence indicates that the detection and functional characterization of antigen-specific T cell subsets within the circulating population may provide a diagnostic indicator of disease and has the potential to predict an individual's response to therapy. In this report, a microarray detection platform that combines grating-coupled surface plasmon resonance imaging (GCSPRI) and grating-coupled surface plasmon coupled emission (SPCE) fluorescence detection modalities was used to detect and characterize CD4+ T cells. The microspot regions of interest (ROIs) printed on the array consisted of immobilized antibodies or peptide loaded MHC monomers (p/MHC) as T cell capture ligands mixed with additional antibodies as cytokine capture ligands covalently bound to the surface of a corrugated gold sensor chip. Using optimized parameters, an unlabelled influenza peptide reactive T cell clone could be detected at a frequency of 0.1% in a mixed T cell sample using GCSPRI. Additionally, after cell binding was quantified, differential TH1 cytokine secretion patterns from a T cell clone cultured under TH1 or TH2 inducing conditions was detected using an SPCE fluorescence based assay. Differences in the secretion patterns of 3 cytokines, characteristic of the inducing conditions, indicated that differences were a consequence of the functional status of the captured cells. A dual mode GCSPRI/SPCE assay can provide a rapid, high content T cell screening/characterization tool that is useful for diagnosing disease, evaluating vaccination efficacy, or assessing responses to immunotherapeutics.
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影响因子：
4.5
作者：
Mishra, KP;Singh, VK;Seth, PK
通讯作者：
Seth, PK
DOI：
10.1073/pnas.0407019102
发表时间：
2005-03-08
影响因子：
11.1
作者：
Stone, JD;Demkowicz, WE;Stern, LJ
通讯作者：
Stern, LJ
DOI：
10.1196/annals.1288.009
发表时间：
2003-01-01
期刊：
IMMUNOLOGY OF DIABETES II: PATHOGENESIS FROM MOUSE TO MAN
影响因子：
--
作者：
Reijonen, H;Kwok, WW;Nepom, GT
通讯作者：
Nepom, GT
影响因子：
8.4
作者：
Schmid, AH;Stanca, SE;Suri, CR
通讯作者：
Suri, CR
DOI：
10.4049/jimmunol.0904180
发表时间：
2010-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Davis JM 3rd;Knutson KL;Strausbauch MA;Crowson CS;Therneau TM;Wettstein PJ;Matteson EL;Gabriel SE
通讯作者：
Gabriel SE