Recurring BALB/c mouse lung inflammatory responses to episodic allergen exposure.
Recurring BALB/c mouse lung inflammatory responses to episodic allergen exposure.
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DOI：
10.1080/15287394.2013.752323
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Bassett DJ
中科院分区：
文献类型：
--
作者：
Wilson SJ;Harmer MJ;Lee RL;Rigden HM;Doyon-Reale NM;Forman KM;Gao X;Lieh-Lai MW;Bassett DJ
这项研究详细介绍了与小鼠间歇性过敏原暴露相关的反复发生的炎症事件的顺序，导致气道高反应性、持续炎症、杯状细胞增生和纤维形成，这些都是慢性哮喘肺部的特征。卵清蛋白 (OVA) 致敏的雌性 Balb/c 小鼠每天接触盐水对照或 OVA 气雾剂 1 小时，每周发作 3 天，持续长达 8 周。使用支气管肺泡灌洗（BAL）和组织胶原酶分散体通过炎症细胞回收来评估肺部炎症。使用免疫组织化学和染色染色方法观察气道粘膜下层和相关血管周围空间内的细胞积聚。气道对乙酰甲胆碱气溶胶的反应性在两周后升高，并在第 4 周和第 8 周后进一步增强至持续水平。尽管到第 8 周时，观察到 OVA 诱导的嗜酸性粒细胞、中性粒细胞和单核巨噬细胞积聚减少，表明反应性减弱，但淋巴细胞的 BAL 恢复仍然升高。气道而非血管周围病变持续存在，并伴有细胞群增殖、上皮杯状细胞增生和胶原沉积增强的证据。在第 1 次、第 2 次和第 4 次 OVA 暴露事件开始之前对肺部炎症细胞含量的检查表明，每次暴露事件中残留 BAL 淋巴细胞和 BAL 以及组织嗜酸性粒细胞的恢复有所增强。尽管组织单核细胞-巨噬细胞数量在每次暴露前恢复到基线，但在第四周后观察到最大水平的积累。这些结果为建立炎症和暴露标准提供了基础，根据该标准，间歇性环境暴露于过敏原可能导致哮喘中肺部重塑。
This study detailed the sequence of recurring inflammatory events associated with episodic allergen exposures of mice resulting in airway hyperreactivity, sustained inflammation, goblet cell hyperplasia, and fibrogenesis that characterize a lung with chronic asthma. Ovalbumin (OVA)-sensitized female Balb/c mice were exposed to saline-control or OVA aerosols for 1hr per day for episodes of 3 days every week for up to 8 weeks. Lung inflammation was assessed by inflammatory cell recoveries using bronchoalveolar lavages (BAL) and tissue collagenase dispersions. Cell accumulations were observed within airway submucosal and associated perivascular spaces using immunohistochemical and tinctorial staining methods. Airway responsiveness to methacholine aerosols were elevated after 2 weeks and further enhanced to a sustained level after the 4th and 8th weeks. Although by the 8th week, diminished OVA-induced accumulations of eosinophils, neutrophils and monocyte-macrophages were observed, suggesting diminished responsiveness, the BAL recovery of lymphocytes remained elevated. Airway but not perivascular lesions persisted with a proliferating cell population, epithelial goblet cell hyperplasia and evidence of enhanced collagen deposition. Examination of lung inflammatory cell content before the onset of the 1st, 2nd and 4th OVA exposure episodes demonstrated enhancements in residual BAL lymphocyte and BAL and tissue eosinophil recoveries with each exposure episode. Although tissue monocyte-macrophage numbers returned to baseline prior to each exposure episode, the greatest level of accumulation was observed after the 4th week. These results provide the basis for establishing the inflammatory and exposure criteria by which episodic environmental exposures to allergen might result in the development of a remodeled lung in asthma.
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影响因子：
15.9
作者：
Evans, CM;Fryer, AD;Costello, RW
通讯作者：
Costello, RW
DOI：
10.1164/ajrccm.156.3.9606031
发表时间：
1997-09-01
影响因子：
24.7
作者：
Hamelmann, E;Schwarze, J;Gelfand, EW
通讯作者：
Gelfand, EW
影响因子：
6.1
作者：
Kumar, RK;Temelkovski, J;Hunter, N
通讯作者：
Hunter, N
影响因子：
8
作者：
通讯作者：
--
影响因子：
10.4
作者：
Gilmour MI;Jaakkola MS;London SJ;Nel AE;Rogers CA
通讯作者：
Rogers CA