Effect of smooth muscle tone on morphometry and residual strain in rat duodenum, jejunum and ileum.
Effect of smooth muscle tone on morphometry and residual strain in rat duodenum, jejunum and ileum.
复制标题
平滑肌张力对大鼠十二指肠、空肠和回肠形态测量和残余应变的影响。
DOI：
10.1016/j.jbiomech.2008.06.017
复制
发表时间：
2008
影响因子：
2.4
通讯作者：
Gregersen,Hans
中科院分区：
文献类型：
--
作者：
Li,Jiang;Zhao,Jingbo;Liao,Donghua;Gregersen,Hans
除了括约肌区域外，难以测量胃肠平滑肌（SM）张力。由于张力影响生物力学特性，本研究的目的是通过研究有和没有肌肉张力的形态计量学和生物力学特性来评估肠道SM张力。从大鼠十二指肠、空肠和回肠切下0.8- 1 mm宽的环。通过切割肠系膜对面的环获得扇区。将环和扇区浸入生理Krebs溶液中以保持音调，并浸入不含Ca++和含EGTA的Krebs溶液中以消除音调。测量或计算粘膜和浆膜表面的周长、面积、圆度和残余应变、开口角和开口角色调/非色调比。张力影响肠段的张开角和残余应变。在三个节段中，有张力的组织节段的张开角均小于无张力的组织节段（P<0.05）。十二指肠、空肠和回肠的张角音调/非音调比值分别为0.40±0.05、0.43±0.06和0.36±0.11，各肠段间差异不显著。十二指肠、空肠粘膜和浆膜层的残余应变在有无SM张力区之间存在显著差异（P<0.05）。肠张力环的管腔面积、肠壁面积、粘膜和浆膜残留应变均呈轴向变化（P <0.001、P<0.05）。总之，肠道的力学性能受到肠道SM张力的影响。通过测量有无SM张力时肠段的张开角和残余应变来评价SM张力。
It is difficult to measure gastrointestinal smooth muscle (SM) tone except in sphincter regions. Since tone affects the biomechanical properties, the aim of the present study was to evaluate intestinal SM tone by studying the morphometry and biomechanical properties with and without muscle tone. Circumferential rings of 0.8–1mm in width were cut from the rat duodenum, jejunum and ileum. Sectors were obtained by cutting the rings opposite to the mesentery. The rings and the sectors were immersed in physiological Krebs solution in order to maintain the tone and into Krebs solution without Ca++and with EGTA to abolish the tone. The circumferences, area, the circularity and residual strain of the mucosal and serosal surfaces, opening angle, and opening angle tone/non-tone ratio were measured or computed. The tone affects the opening angle and residual strain in the intestinal sectors. The opening angle in the tissue sectors with tone was smaller (P<0.05) than those without tone in all three segments. The opening angle tone/non-tone ratio was 0.40±0.05, 0.43±0.06 and 0.36±0.11 for duodenum, jejunum and ileum, respectively, and did not differ among the three intestinal segments. The residual strain between sectors with and without SM tone differed in duodenal and jejunal mucosa and in the serosa of all three segments (P<0.05). The intestinal rings with tone showed axial variation for luminal area (P<0.001), for wall area (P<0.05), and for the mucosal and serosal residual strains (P<0.05). In conclusion, the intestinal mechanical properties are affected by intestinal SM tone. The tone can be evaluated by measuring the opening angle and residual strains of sectors in intestinal segments with and without SM tone.
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影响因子：
--
作者：
GREGERSEN, H;ORVAR, K;CHRISTENSEN, J
通讯作者：
CHRISTENSEN, J
影响因子：
4.3
作者：
James B Smith;Jingbo Zhao;Y. Dou;H. Gregersen
通讯作者：
H. Gregersen
胆碱能激动剂对啮齿动物近端和远端小肠肌肉的影响。
影响因子：
29.4
作者：
Nowak,TV;Harrington,B
通讯作者：
Harrington,B
DOI：
10.1007/978-88-470-1405-3_42
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Arieh Riskin;Carlo Agostoni;R. Shamir
通讯作者：
Arieh Riskin;Carlo Agostoni;R. Shamir
影响因子：
--
作者：
EHRLEIN, HJ;SCHEMANN, M;SIEGLE, ML
通讯作者：
SIEGLE, ML