Characteristic Gene Expression Profiles of Human Fibroblasts and Breast Cancer Cells in a Newly Developed Bilateral Coculture System.
Characteristic Gene Expression Profiles of Human Fibroblasts and Breast Cancer Cells in a Newly Developed Bilateral Coculture System.
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新开发的双侧共培养系统中人成纤维细胞和乳腺癌细胞的特征基因表达谱。
DOI：
10.1155/2015/960840
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发表时间：
2015
影响因子：
--
通讯作者：
Shimizu K
中科院分区：
文献类型：
--
作者：
Ueno T;Utsumi J;Toi M;Shimizu K
癌细胞的微环境与癌症的发展和进展有关。癌症相关的成纤维细胞构成微环境的主要基质成分。为了分析癌细胞和成纤维细胞之间的相互作用，我们开发了一种新的双边共培养系统，使用双面微孔胶原膜。将人正常皮肤成纤维细胞与三种不同的人乳腺癌细胞系：MCF-7、SK-BR-3和HCC 1937共培养。共培养后，分别从癌细胞和成纤维细胞中提取mRNA，并将其应用于微阵列的转录组学分析。前500个通常上调或下调的基因通过使用MetaCore功能分析的富集功能分析来表征。大多数在癌细胞中上调的基因在成纤维细胞中下调，而大多数在癌细胞中下调的基因在成纤维细胞中上调，表明mRNA表达的变化模式在癌细胞和成纤维细胞之间是相互的。在共培养中，乳腺癌细胞通常增加与有丝分裂反应和TCA途径相关的基因，而成纤维细胞增加与糖代谢相关的基因，包括糖酵解、糖原生成和葡萄糖转运，表明成纤维细胞通过提供能量来源支持癌细胞增殖。我们建议，使用胶原膜的双边共培养系统是有用的，通过模拟体内肿瘤微环境来研究癌细胞和基质细胞之间的相互作用。
The microenvironment of cancer cells has been implicated in cancer development and progression. Cancer-associated fibroblast constitutes a major stromal component of the microenvironment. To analyze interaction between cancer cells and fibroblasts, we have developed a new bilateral coculture system using a two-sided microporous collagen membrane. Human normal skin fibroblasts were cocultured with three different human breast cancer cell lines: MCF-7, SK-BR-3, and HCC1937. After coculture, mRNA was extracted separately from cancer cells and fibroblasts and applied to transcriptomic analysis with microarray. Top 500 commonly up- or downregulated genes were characterized by enrichment functional analysis using MetaCore Functional Analysis. Most of the genes upregulated in cancer cells were downregulated in fibroblasts while most of the genes downregulated in cancer cells were upregulated in fibroblasts, indicating that changing patterns of mRNA expression were reciprocal between cancer cells and fibroblasts. In coculture, breast cancer cells commonly increased genes related to mitotic response and TCA pathway while fibroblasts increased genes related to carbohydrate metabolism including glycolysis, glycogenesis, and glucose transport, indicating that fibroblasts support cancer cell proliferation by supplying energy sources. We propose that the bilateral coculture system using collagen membrane is useful to study interactions between cancer cells and stromal cells by mimicking in vivo tumor microenvironment.
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影响因子：
--
作者：
Casbas-Hernandez P;Fleming JM;Troester MA
通讯作者：
Troester MA
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
11.2
作者：
Liu S;Ginestier C;Ou SJ;Clouthier SG;Patel SH;Monville F;Korkaya H;Heath A;Dutcher J;Kleer CG;Jung Y;Dontu G;Taichman R;Wicha MS
通讯作者：
Wicha MS
影响因子：
4.8
作者：
Buess, Martin;Rajski, Michal;Rochlitz, Christoph
通讯作者：
Rochlitz, Christoph
影响因子：
--
作者：
Costa Santos, Rosangela Portilho;Benvenuti, Ticiana Thomazine;Azevedo Koike Folgueira, Maria Aparecida
通讯作者：
Azevedo Koike Folgueira, Maria Aparecida