Multi-species single-cell transcriptomic analysis of ocular compartment regulons.
Multi-species single-cell transcriptomic analysis of ocular compartment regulons.
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DOI：
10.1038/s41467-021-25968-8
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发表时间：
2021-09-28
影响因子：
16.6
通讯作者：
Loh YH
中科院分区：
文献类型：
--
作者：
Gautam P;Hamashima K;Chen Y;Zeng Y;Makovoz B;Parikh BH;Lee HY;Lau KA;Su X;Wong RCB;Chan WK;Li H;Blenkinsop TA;Loh YH
通过单细胞RNA测序研究，视网膜是多种物种中广泛分布的组织。然而，缺乏跨物种视网膜的综合研究。在这里，我们构建了人类和猪眼隔间的第一个单细胞图谱，并研究了视网膜的物种间差异。除此之外，我们还鉴定了虹膜组织中存在的推定成体干细胞。我们还绘制了一幅与眼部疾病有关的基因疾病图。此外，我们探索了不同细胞群的调控，包括转录因子和受体-配体相互作用，并揭示了眼细胞类型之间独特的定向信号传导。此外，我们研究了脊椎动物和斑马鱼之间的规则保护，以确定共同的核心因素。在这里，我们展示了KLF7基因在视网膜神经节细胞分化过程中的表达扰动，并得出结论，它在视网膜神经节细胞的成熟中起着重要作用。全面分析眼各组织间的眼网络是理解健康和疾病中的眼生理的必要条件。在这里，作者展示了一个由角膜、虹膜、睫状体、神经视网膜、视网膜色素上皮和脉络膜细胞组成的多物种单细胞转录组图谱。
The retina is a widely profiled tissue in multiple species by single-cell RNA sequencing studies. However, integrative research of the retina across species is lacking. Here, we construct the first single-cell atlas of the human and porcine ocular compartments and study inter-species differences in the retina. In addition to that, we identify putative adult stem cells present in the iris tissue. We also create a disease map of genes involved in eye disorders across compartments of the eye. Furthermore, we probe the regulons of different cell populations, which include transcription factors and receptor-ligand interactions and reveal unique directional signalling between ocular cell types. In addition, we study conservation of regulons across vertebrates and zebrafish to identify common core factors. Here, we show perturbation of KLF7 gene expression during retinal ganglion cells differentiation and conclude that it plays a significant role in the maturation of retinal ganglion cells. A comprehensive analysis of the ocular networks among various tissues is necessary to understand eye physiology in health and disease. Here the authors present a multi-species single-cell transcriptomic atlas consisting of cells of the cornea, iris, ciliary body, neural retina, retinal pigmented epithelium, and choroid.
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影响因子：
1.8
作者：
McMahon AR;Merzdorf CS
通讯作者：
Merzdorf CS
影响因子：
13.6
作者：
Brodie-Kommit J;Clark BS;Shi Q;Shiau F;Kim DW;Langel J;Sheely C;Ruzycki PA;Fries M;Javed A;Cayouette M;Schmidt T;Badea T;Glaser T;Zhao H;Singer J;Blackshaw S;Hattar S
通讯作者：
Hattar S
影响因子：
4.4
作者：
Hornan, Daniel M.;Peirson, Stuart N.;Webster, Andrew R.
通讯作者：
Webster, Andrew R.
影响因子：
9.8
作者：
Daniszewski M;Senabouth A;Nguyen QH;Crombie DE;Lukowski SW;Kulkarni T;Sluch VM;Jabbari JS;Chamling X;Zack DJ;Pébay A;Powell JE;Hewitt AW
通讯作者：
Hewitt AW
影响因子：
64.8
作者：
La Manno G;Soldatov R;Zeisel A;Braun E;Hochgerner H;Petukhov V;Lidschreiber K;Kastriti ME;Lönnerberg P;Furlan A;Fan J;Borm LE;Liu Z;van Bruggen D;Guo J;He X;Barker R;Sundström E;Castelo-Branco G;Cramer P;Adameyko I;Linnarsson S;Kharchenko PV
通讯作者：
Kharchenko PV