Genome-Wide Identification, Expression, and Functional Analysis of the Sugar Transporter Gene Family in Cassava (Manihot esculenta).
Genome-Wide Identification, Expression, and Functional Analysis of the Sugar Transporter Gene Family in Cassava (Manihot esculenta).
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木薯 (Manihot esculenta) 糖转运蛋白基因家族的全基因组鉴定、表达和功能分析
DOI：
10.3390/ijms19040987
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发表时间：
2018-03-26
影响因子：
5.6
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Liu Q;Dang H;Chen Z;Wu J;Chen Y;Chen S;Luo L
糖转运蛋白 (STP) 基因家族编码包含 12 个跨膜结构域的单糖转运蛋白，属于主要促进子超家族。 STP 基因在单糖分布中发挥着关键作用，并参与多种植物代谢过程。为了研究STP在木薯（Manihot esculenta）块根生长中的潜在作用，本研究对STP基因家族进行了全基因组鉴定和表达以及功能分析。从木薯基因组中鉴定出总共 20 个包含 Sugar_tr 保守基序的 MeSTP 基因 (MeSTP1-20)，这些基因可以在系统发育树中进一步分为四个不同的组。利用RNA-seq数据探索的MeSTP基因的表达谱显示，大多数MeSTP基因表现出组织特异性表达，20个MeSTP基因中有15个主要在木薯的早期贮藏根中表达。 qRT-PCR分析进一步证实，大多数MeSTPs在生长30天和40天后在根中表现出较高的表达，表明这些基因可能参与块根的早期生长。尽管所有 MeSTP 蛋白均表现出质膜定位，但通过对单糖摄取缺陷的酵母（酿酒酵母）突变体进行互补分析，发现单糖转运活性存在差异。其中，MeSTP2、MeSTP15和MeSTP19能够有效补充酵母突变体中五种单糖的摄取，而MeSTP3和MeSTP16仅在含有半乳糖的培养基上生长，表明这两种MeSTP蛋白是半乳糖特异性转运蛋白。这项研究为 MeSTP 在早期块根生长中的潜在功能提供了重要的见解，这可能涉及单糖分布的调节。
The sugar transporter (STP) gene family encodes monosaccharide transporters that contain 12 transmembrane domains and belong to the major facilitator superfamily. STP genes play critical roles in monosaccharide distribution and participate in diverse plant metabolic processes. To investigate the potential roles of STPs in cassava (Manihot esculenta) tuber root growth, genome-wide identification and expression and functional analyses of the STP gene family were performed in this study. A total of 20 MeSTP genes (MeSTP1–20) containing the Sugar_tr conserved motifs were identified from the cassava genome, which could be further classified into four distinct groups in the phylogenetic tree. The expression profiles of the MeSTP genes explored using RNA-seq data showed that most of the MeSTP genes exhibited tissue-specific expression, and 15 out of 20 MeSTP genes were mainly expressed in the early storage root of cassava. qRT-PCR analysis further confirmed that most of the MeSTPs displayed higher expression in roots after 30 and 40 days of growth, suggesting that these genes may be involved in the early growth of tuber roots. Although all the MeSTP proteins exhibited plasma membrane localization, variations in monosaccharide transport activity were found through a complementation analysis in a yeast (Saccharomyces cerevisiae) mutant, defective in monosaccharide uptake. Among them, MeSTP2, MeSTP15, and MeSTP19 were able to efficiently complement the uptake of five monosaccharides in the yeast mutant, while MeSTP3 and MeSTP16 only grew on medium containing galactose, suggesting that these two MeSTP proteins are transporters specific for galactose. This study provides significant insights into the potential functions of MeSTPs in early tuber root growth, which possibly involves the regulation of monosaccharide distribution.
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DOI：
10.1038/nsb821
发表时间：
2002-08-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Hirai, T;Heymann, JAW;Subramaniam, S
通讯作者：
Subramaniam, S
影响因子：
4.4
作者：
An D;Yang J;Zhang P
通讯作者：
Zhang P
影响因子：
7.3
作者：
Büttner, M;Truernit, E;Sauer, N
通讯作者：
Sauer, N
影响因子：
10.7
作者：
Johnson, Deborah A.;Thomas, Michael A.
通讯作者：
Thomas, Michael A.
影响因子：
46.9
作者：
Bredeson, Jessen V.;Lyons, Jessica B.;Rokhsar, Daniel S.
通讯作者：
Rokhsar, Daniel S.