Higher biomass accumulation by increasing phosphoribosylpyrophosphate synthetase activity in Arabidopsis thaliana and Nicotiana tabacum.
Higher biomass accumulation by increasing phosphoribosylpyrophosphate synthetase activity in Arabidopsis thaliana and Nicotiana tabacum.
复制标题
通过增加拟南芥和尼古丁塔巴库姆的磷酸糖基磷酸合成酶活性来增加生物量的积累。
DOI：
10.1111/j.1467-7652.2007.00314.x
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发表时间：
2008-04
影响因子：
13.8
通讯作者：
Zrenner, Rita
中科院分区：
文献类型：
--
作者：
Koslowsky, Silke;Riegler, Heike;Bergmueller, Eveline;Zrenner, Rita
植物能够产生发育和生长所需的所有有机化合物。由于发育过程和代谢途径使用共同的资源库，因此可以假设代谢物在生长、防御化合物的生产和储存产品之间的分布受到严格调节。采用转基因方法研究了提供关键中间体磷酸核糖焦磷酸 (PRPP) 对于模型植物拟南芥和烟草中植物生长和生物量积累的重要性。为此，编码 PRPP 合成酶 (PRS) 或同一基因突变变体的棉阿什比亚基因在组成型启动子的控制下过表达。结果表明，在不同的标准化生长条件下，拟南芥或红花中PRS活性的增加导致生物量积累的大幅增加。生长促进伴随着糖和其他代谢物含量的显着变化。这项研究提供的证据表明，PRPP 的供应共同限制了生长速度，并且对旨在增加植物生物量作为替代可再生能源的生物技术策略具有明显的影响。
Plants are able to produce all the organic compounds required for development and growth. As developmental processes and metabolic pathways use a common resource pool, the tight regulation of the distribution of metabolites between growth, production of defence compounds and storage products can be assumed. A transgenic approach was used to investigate the importance of supplying the key intermediate phosphoribosylpyrophosphate (PRPP) for plant growth and biomass accumulation in the model plant Arabidopsis thaliana and in Nicotiana tabacum. For this purpose, the Ashbya gossypii genes coding for either PRPP synthetase (PRS) or a mutated variant of the same gene were over-expressed under the control of a constitutive promoter. It was shown that increased PRS activity in A. thaliana or N. tabacum leads to a substantial increase in biomass accumulation under different standardized growth conditions. Growth enhancement was accompanied by significant changes in the amount of sugars and other metabolites. This study provides evidence that the supply of PRPP co-limits growth rates, and has obvious implications for biotechnological strategies aiming to increase plant biomass as an alternative renewable energy source.
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影响因子：
11.4
作者：
ROSAHL, S;SCHELL, J;WILLMITZER, L
通讯作者：
WILLMITZER, L
影响因子：
7.4
作者：
Scheible, WR;Morcuende, R;Stitt, M
通讯作者：
Stitt, M
影响因子：
6.9
作者：
Baldet, P;Hernould, M;Rothan, C
通讯作者：
Rothan, C
影响因子：
3.2
作者：
BENNING, C;SOMERVILLE, CR
通讯作者：
SOMERVILLE, CR
影响因子：
4.3
作者：
DANCER, JE;APREES, T
通讯作者：
APREES, T