A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement.
A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement.
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DOI：
10.1007/s00122-018-3102-x
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Tsujimoto H
中科院分区：
文献类型：
--
作者：
Gorafi YSA;Kim JS;Elbashir AAE;Tsujimoto H
本研究所建立的多重合成衍生物平台将为粗山羊草性状和基因在小麦育种中的有效利用提供契机。从野生近缘种中引入基因是增加遗传多样性和发现小麦改良所需的新等位基因的最佳选择。一个含有二倍体小麦祖先节节麦基因组片段的群体。在面包小麦（Triticum aestivum L.）通过43个人工合成小麦品系与普通小麦品种Norin 61杂交和回交，我们将这一群体命名为多重合成衍生物（MSD）。为了验证该群体对小麦育种和遗传研究的适用性，我们随机选择了400个MSD系，并通过使用多样性阵列技术测序标记对它们进行基因分型。我们得分黑颖作为一个质量性状和抽穗期在两个环境中在苏丹作为一个数量性状。我们的研究结果表明，高的遗传多样性和较少的重组，这是预期从人口的性质。全基因组关联分析（GWA）显示，在第1D染色体短臂上有一个QTL与已报道的等位基因不同，表明默沙东群体的黑颖是由同一位点上的新等位基因控制的。在抽穗期上，GWA分析发现，在2A、2B和2D染色体短臂上有3个QTL，在5A和5D染色体长臂上有2个QTL。使用MSD群体，这代表了43 Ae的多样性。通过对代表其大部分自然生境的节节麦种质、QTL或基因以及所需表型（如耐旱性、耐热性和耐盐性）进行鉴定和选择，可为小麦育种提供参考。 本文的在线版本（10.1007/s 00122 -018-3102-x）包含补充材料，可供授权用户使用。
The multiple synthetic derivatives platform described in this study will provide an opportunity for effective utilization of Aegilops tauschii traits and genes for wheat breeding. Introducing genes from wild relatives is the best option to increase genetic diversity and discover new alleles necessary for wheat improvement. A population harboring genomic fragments from the diploid wheat progenitor Aegilops tauschii Coss. in the background of bread wheat (Triticum aestivum L.) was developed by crossing and backcrossing 43 synthetic wheat lines with the common wheat cultivar Norin 61. We named this population multiple synthetic derivatives (MSD). To validate the suitability of this population for wheat breeding and genetic studies, we randomly selected 400 MSD lines and genotyped them by using Diversity Array Technology sequencing markers. We scored black glume as a qualitative trait and heading time in two environments in Sudan as a quantitative trait. Our results showed high genetic diversity and less recombination which is expected from the nature of the population. Genome-wide association (GWA) analysis showed one QTL at the short arm of chromosome 1D different from those alleles reported previously indicating that black glume in the MSD population is controlled by new allele at the same locus. For heading time, from the two environments, GWA analysis revealed three QTLs on the short arms of chromosomes 2A, 2B and 2D and two on the long arms of chromosomes 5A and 5D. Using the MSD population, which represents the diversity of 43 Ae. tauschii accessions representing most of its natural habitat, QTLs or genes and desired phenotypes (such as drought, heat and salinity tolerance) could be identified and selected for utilization in wheat breeding. The online version of this article (10.1007/s00122-018-3102-x) contains supplementary material, which is available to authorized users.
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DOI：
10.1534/g3.114.012963
发表时间：
2014-09-18
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Mackay IJ;Bansept-Basler P;Barber T;Bentley AR;Cockram J;Gosman N;Greenland AJ;Horsnell R;Howells R;O'Sullivan DM;Rose GA;Howell PJ
通讯作者：
Howell PJ
影响因子：
5.6
作者：
Kurahashi Y;Terashima A;Takumi S
通讯作者：
Takumi S
影响因子：
9.4
作者：
Guo, Zhiai;Song, Yanxia;Jia, Jizeng
通讯作者：
Jia, Jizeng
影响因子：
2.4
作者：
Elbashir AAE;Gorafi YSA;Tahir ISA;Kim JS;Tsujimoto H
通讯作者：
Tsujimoto H
影响因子：
5.4
作者：
Börner, A;Schumann, E;Weber, WE
通讯作者：
Weber, WE