Pathogens in ticks collected from dogs in Berlin/Brandenburg, Germany.
Pathogens in ticks collected from dogs in Berlin/Brandenburg, Germany.
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从柏林/德国勃兰登堡的狗收集的壁虱中的病原体。
DOI：
10.1186/s13071-014-0535-1
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发表时间：
2014-12-02
影响因子：
3.2
通讯作者：
von Samson-Himmelstjerna G
中科院分区：
文献类型：
--
作者：
Schreiber C;Krücken J;Beck S;Maaz D;Pachnicke S;Krieger K;Gross M;Kohn B;von Samson-Himmelstjerna G
蜱虫传播的疾病是人类和狗的主要健康风险。除了收集和分析探索蜱虫外，分析宿主相关蜱虫是否存在病原体是深入了解蜱传疾病传播模式的一种有价值的方法。从生活在柏林/勃兰登堡地区的犬中采集蜱虫。检查了三种蜱种属篦麻硬蜱、六角硬蜱和网纹革蜱中是否存在巴贝虫属，疏螺旋体属，立克次体属和无形体科。常规PCR测序用于病原体检测和表征。巴贝虫属在I.蓖麻和I. hexagonus，分别。测序结果显示，存在的Bababelioti，Bababelicapreoli和Bababelivenatorum。D.网壳绦虫不含犬巴贝虫。立克次体属61%的I. ricinus，占I. hexagonus和D.网状的在D. reticulatus和I.六边形、瑞士立克次体在I.蓖麻和I.六角立克次体和摩纳哥立克次体。六边形嗜吞噬细胞无浆体和Neoehrlichia mikurensis无浆体在I.蓖麻（6.5%）和I.六角型（3.9%和5.9%）。疏螺旋体属11.6%的I.蓖麻和I.六边形随后的基因种分析显示阿氏疏螺旋体、伽氏疏螺旋体、狭义伯氏疏螺旋体和宫本疏螺旋体。20%的感染者同时存在一种以上的病原体。ricinus和I. hexagonus中任一病原体的总检出率为65%。蓖麻，占59%; hexagonus和D.网状的其中A.检测到的嗜吞噬细胞菌也含有立克次体的风险显著增加。携带病原体的蜱比不存在任何病原体的蜱具有显著更高的鳞甲指数。蜱中潜在的人类或犬病原体的频率相当大，检测到所有四组病原体的DNA。盾指数的差异可能表明，病原体经常被蜱在柏林/勃兰登堡的狗喂养时。本文的在线版本（doi：10.1186/s13071-014-0535-1）包含补充材料，可供授权用户使用。
Tick-borne diseases are a major health risk for humans and dogs. In addition to collection and analysis of questing ticks, analysis of host-associated ticks for the presence of pathogens is a valuable method to gain insight into transmission patterns of tick-borne diseases. Ticks were collected from dogs living in the Berlin/Brandenburg area. The three tick species Ixodes ricinus, Ixodes hexagonus and Dermacentor reticulatus were examined for the presence of Babesia spp., Borrelia spp., Rickettsia spp. and Anaplasmataceae. Conventional PCR followed by sequencing was used for pathogen detection and characterization. Babesia spp. were found in 2.5% and 3% of I. ricinus and I. hexagonus, respectively. Sequencing revealed the presence of Babesia microti, Babesia capreoli and Babesia venatorum. D. reticulatus were free of Babesia canis. Rickettsia spp. were detected in 61% of I. ricinus, 44% of I. hexagonus and 39% of D. reticulatus. Specifically detected were Rickettsia raoulti in D. reticulatus and I. hexagonus, Rickettsia helvetica in I. ricinus and I. hexagonus and Rickettsia monacensis in I. hexagonus. Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis have been reported previously in I. ricinus (6.5% and 4.3%, respectively) and I. hexagonus (3.9% and 5.9%). Borrelia spp. were found in 11.6% of I. ricinus and 11.2% of I. hexagonus. Subsequent genospecies analysis revealed Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto and Borrelia miyamotoi. Simultanous presence of more than one pathogen was found in 20% of I. ricinus and in 59% of I. hexagonus whereas the total frequency of any pathogen was 65% in I. ricinus, 59% in I. hexagonus and 64% in D. reticulatus. Ticks in which A. phagocytophilum was detected had a significantly increased risk of also containing Rickettsia. Ticks harbouring a pathogen had significantly higher scutal indices than ticks without presence of any pathogen. Frequencies of potential human or canine pathogens in ticks were considerable and DNA of all four groups of pathogens was detected. Differences in scutal indices might suggest that pathogens are frequently taken up by ticks when feeding on dogs in Berlin/Brandenburg. The online version of this article (doi:10.1186/s13071-014-0535-1) contains supplementary material, which is available to authorized users.
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影响因子：
11.8
作者：
Becker CA;Bouju-Albert A;Jouglin M;Chauvin A;Malandrin L
通讯作者：
Malandrin L
DOI：
10.1007/s10096-007-0333-1
发表时间：
2007-08-01
影响因子：
4.5
作者：
Hildebrandt, A.;Hunfeld, K.-P.;Straube, E.
通讯作者：
Straube, E.
影响因子：
4.1
作者：
Dautel, Hans;Dippel, Cornelia;Schettler, Elvira
通讯作者：
Schettler, Elvira
影响因子：
9.4
作者：
Duh, D;Petrovec, M;Avsic-Zupanc, T
通讯作者：
Avsic-Zupanc, T
影响因子：
5
作者：
Falco, RC;Fish, D;Piesman, J
通讯作者：
Piesman, J