Porcine deltacoronavirus infection: Etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis.
Porcine deltacoronavirus infection: Etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis.
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DOI：
10.1016/j.virusres.2016.04.009
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发表时间：
2016-12-02
期刊：
影响因子：
5
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Jung K;Hu H;Saif LJ
猪DeltaCoronaVirus(PDCoV)是一种新型猪肠道致病冠状病毒。介绍了PDCoV的病原学和分子流行病学。对PDCoV分离和繁殖的细胞培养方法进行了研究。本文对PDCoV的致病机理和致病机理进行了综述。猪DeltaCoronaVirus(PDCoV)是由冠状病毒科DeltaCoronaVirus(DeltaCoronaVirus)科的DeltaCoronaVirus(DeltaCoronaVirus)引起的一种新的猪肠源性冠状病毒，可引起血清阴性新生仔猪的急性腹泻/呕吐、脱水和死亡。PDCoV腹泻于2014年初在美国首次报告，与猪流行性腹泻病毒(PEDV)(冠状病毒科，阿尔法冠状病毒属)同时传播。PDCoV在猪身上的起源以及它的突然出现或传入美国的途径仍不清楚。在美国，自2013-2014年以来，新出现的PDCoV和PEDV在全国范围内传播，导致大量生猪死亡和重大经济影响。目前的美国PDCoV毒株是肠源性的，可感染整个小肠和大肠的绒毛上皮细胞，尽管空肠和回肠是主要的感染部位。与PEDV感染类似，PDCoV感染也会导致急性严重萎缩性肠炎，并伴有一过性病毒血症(病毒RNA)，导致严重腹泻和/或呕吐，随后脱水成为哺乳仔猪的潜在死亡原因。目前，对PDCoV、PEDV和传染性胃肠炎病毒(TGEV)的鉴别诊断是控制美国猪病毒性腹泻的关键。细胞培养适应的美国PDCoV(TC-PDCoV)毒株已经由我们和其他几个实验室分离和繁殖。TC-PDCoV毒株可用于建立血清学检测方法和评估连续细胞培养传代是否能减弱TC-PDCoV作为疫苗候选毒株的作用。目前需要全面了解流行的PDCoV毒株的发病机制和流行病学，以在受影响地区预防和控制疾病，并开发有效的疫苗。本文就PDCoV感染的病原学、细胞培养分离与繁殖、分子流行病学、发病机制及致病机制等方面的研究进展作一综述。
Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus. The etiology and molecular epidemiology of PDCoV are described. The cell culture for PDCoV isolation and propagation are demonstrated. The disease mechanisms and pathogenesis of PDCoV are reviewed. Porcine deltacoronavirus (PDCoV) (family Coronaviridae, genus Deltacoronavirus) is a novel swine enteropathogenic coronavirus that causes acute diarrhea/vomiting, dehydration and mortality in seronegative neonatal piglets. PDCoV diarrhea was first reported in the US in early 2014, concurrently with co-circulation of porcine epidemic diarrhea virus (PEDV) (family Coronaviridae, genus Alphacoronavirus). The origin of PDCoV in pigs and also its sudden emergence or route of introduction into the US still remains unclear. In the US, since 2013–2014, the newly emerged PDCoV and PEDV have spread nationwide, causing a high number of pig deaths and significant economic impacts. The current US PDCoV strains are enteropathogenic and infect villous epithelial cells of the entire small and large intestines although the jejunum and ileum are the primary sites of infection. Similar to PEDV infections, PDCoV infections also cause acute, severe atrophic enteritis accompanied by transient viremia (viral RNA) that leads to severe diarrhea and/or vomiting, followed by dehydration as the potential cause of death in nursing piglets. At present, differential diagnosis of PDCoV, PEDV, and transmissible gastroenteritis virus (TGEV) is essential to control viral diarrheas in US swine. Cell culture-adapted US PDCoV (TC-PDCoV) strains have been isolated and propagated by us and in several other laboratories. TC-PDCoV strains will be useful to develop serologic assays and to evaluate if serial cell-culture passage attenuates TC-PDCoV as a potential vaccine candidate strain. A comprehensive understanding of the pathogenesis and epidemiology of epidemic PDCoV strains is currently needed to prevent and control the disease in affected regions and to develop an effective vaccine. This review focuses on the etiology, cell culture isolation and propagation, molecular epidemiology, disease mechanisms and pathogenesis of PDCoV infection.
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影响因子：
3.3
作者：
Jung K;Hu H;Saif LJ
通讯作者：
Saif LJ
影响因子：
3.7
作者：
Chen Q;Gauger P;Stafne M;Thomas J;Arruda P;Burrough E;Madson D;Brodie J;Magstadt D;Derscheid R;Welch M;Zhang J
通讯作者：
Zhang J
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DOI：
10.3390/v7102891
发表时间：
2015-10-23
期刊：
Viruses
影响因子：
--
作者：
Chen F;Zhu Y;Wu M;Ku X;Ye S;Li Z;Guo X;He Q
通讯作者：
He Q
影响因子：
1.8
作者：
de Arriba ML;Carvajal A;Pozo J;Rubio P
通讯作者：
Rubio P
氨基肽酶N是肠道病毒冠状病毒TGEV的主要受体。
影响因子：
64.8
作者：
Delmas B;Gelfi J;L'Haridon R;Vogel LK;Sjöström H;Norén O;Laude H
通讯作者：
Laude H