MicroRNAs of Bombyx mori identified by Solexa sequencing.
MicroRNAs of Bombyx mori identified by Solexa sequencing.
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DOI：
10.1186/1471-2164-11-148
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发表时间：
2010-03-03
期刊：
影响因子：
4.4
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Liu S;Li D;Li Q;Zhao P;Xiang Z;Xia Q
MicroRNA(MiRNA)和其他小的调控RNA有助于调节大量的细胞过程。本研究对家蚕前、中、后丝腺和全身3个小RNA文库进行了测序，以期扩大家蚕miRNAs谱库，探索丝腺片段间miRNAs的转录差异。借助于大规模Solexa测序技术，我们验证了257个独特的miRNA基因，其中包括202个新基因和55个已报道的基因，对应于家蚕基因组中的324个基因座。对30多个已知的家蚕miRNAs的序列组成和长度进行了进一步的修正。一些读取来自先前报道的两个miRNAs(BMO-miR-1920和miR-1921)前体的环区。有趣的是，大多数新发现的miRNAs是家蚕特有的，23个独特的miRNAs在无脊椎动物和脊椎动物中广泛保守，13个独特的miRNAs仅限于无脊椎动物，32个仅限于昆虫。我们在家蚕基因组中鉴定了24个紧密定位的簇和45个平行的miRNAs。然而，序列标签表明，并列或簇不是协调转录和积累的先决条件。大多数家蚕特异的miRNAs位于转座元件中，并且在前、中、后丝腺的丰度上存在显著差异。保守的分析表明，miRNAs可以作为系统发育标记并在进化信号中发挥作用。这些新发现的miRNAs极大地丰富了昆虫的miRNAs谱系，并为深入研究miRNA的进化、生物发生和在昆虫中的表达提供了新的视角。MiRNAs在前、中、后丝腺中的差异表达支持它们作为新的水平参与家蚕丝腺的调控。
MicroRNA (miRNA) and other small regulatory RNAs contribute to the modulation of a large number of cellular processes. We sequenced three small RNA libraries prepared from the whole body, and the anterior-middle and posterior silk glands of Bombyx mori, with a view to expanding the repertoire of silkworm miRNAs and exploring transcriptional differences in miRNAs between segments of the silk gland. With the aid of large-scale Solexa sequencing technology, we validated 257 unique miRNA genes, including 202 novel and 55 previously reported genes, corresponding to 324 loci in the silkworm genome. Over 30 known silkworm miRNAs were further corrected in their sequence constitutes and length. A number of reads originated from the loop regions of the precursors of two previously reported miRNAs (bmo-miR-1920 and miR-1921). Interestingly, the majority of the newly identified miRNAs were silkworm-specific, 23 unique miRNAs were widely conserved from invertebrates to vertebrates, 13 unique miRNAs were limited to invertebrates, and 32 were confined to insects. We identified 24 closely positioned clusters and 45 paralogs of miRNAs in the silkworm genome. However, sequence tags showed that paralogs or clusters were not prerequisites for coordinated transcription and accumulation. The majority of silkworm-specific miRNAs were located in transposable elements, and displayed significant differences in abundance between the anterior-middle and posterior silk gland. Conservative analysis revealed that miRNAs can serve as phylogenetic markers and function in evolutionary signaling. The newly identified miRNAs greatly enrich the repertoire of insect miRNAs, and provide insights into miRNA evolution, biogenesis, and expression in insects. The differential expression of miRNAs in the anterior-middle and posterior silk glands supports their involvement as new levels in the regulation of the silkworm silk gland.
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影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
家蚕中保守 microRNA 的鉴定以及异源系统中 microRNA 对丝心蛋白 L 链产生的调节。
影响因子：
3.8
作者：
Cao, Jun;Tong, Chuanzhou;Jin, Yongfeng
通讯作者：
Jin, Yongfeng
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
影响因子：
14.9
作者：
Hinas, Andrea;Reimegard, Johan;Soderbom, Fredrik
通讯作者：
Soderbom, Fredrik
影响因子：
56.9
作者：
Farh, KKH;Grimson, A;Bartel, DP
通讯作者：
Bartel, DP