Silicon dioxide nanoparticle exposure affects small intestine function in an in vitro model.
Silicon dioxide nanoparticle exposure affects small intestine function in an in vitro model.
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DOI：
10.1080/17435390.2018.1463407
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发表时间：
2018-06
期刊：
影响因子：
5
通讯作者：
Mahler GJ
中科院分区：
文献类型：
--
作者：
Guo Z;Martucci NJ;Liu Y;Yoo E;Tako E;Mahler GJ
使用纳米材料来增强食品的特性和改善口服药物的传递已经变得很常见，但这些摄入的纳米材料对健康的潜在影响仍然不清楚。本研究的目的是表征食品和食品包装中常用的二氧化硅纳米颗粒(NP)的性质，并调查生理上真实剂量的SiO_2纳米颗粒对胃肠道健康和功能的影响。在这项工作中，使用了Caco-2和HT29-MTX共培养的体外模型，它们代表了吸收细胞和杯状细胞。模型在急性(4小时)和慢性(5天)时间段内暴露于具有良好特征的二氧化硅NP。暴露于SiO_2 NP显著影响铁、锌、葡萄糖和脂肪养分的吸收。刷状缘膜的肠道碱性磷酸酶活性随纳米SiO_2的加入而增强。通过跨上皮电阻法测量的肠道上皮屏障功能在慢性暴露时显著降低。基因表达和氧化应激形成分析表明，NP改变了营养转运蛋白的表达水平，产生了活性氧物种，并启动了促炎信号。暴露于SiO_2-NP使肠道微绒毛数量减少，从而破坏了刷状缘膜，从而减少了养分吸收的表面积。在体外模型中，生理相关剂量的二氧化硅NP暴露最终导致不良后果。
The use of nanomaterials to enhance properties of food and improve delivery of orally-administered drugs has become common, but the potential health effects of these ingested nanomaterials remains unknown. The goal of this study is to characterize the properties of silicon dioxide (SiO2) nanoparticles (NP) that are commonly used in food and food packaging, and to investigate the effects of physiologically realistic doses of SiO2 NP on gastrointestinal health and function. In this work an in vitro model composed of Caco-2 and HT29-MTX co-cultures, which represent absorptive and goblet cells, was used. The model was exposed to well-characterized SiO2 NP for acute (4 hours) and chronic (5 days) time periods. SiO2 NP exposure significantly affected iron, zinc, glucose, and lipid nutrient absorption. Brush border membrane intestinal alkaline phosphatase activity was increased in response to nano-SiO2. The barrier function of the intestinal epithelium, as measured by transepithelial electrical resistance, was significantly decreased in response to chronic exposure. Gene expression and oxidative stress formation analysis showed NP altered the expression levels of nutrient transport proteins, generated reactive oxygen species, and initiated pro-inflammatory signaling. SiO2 NP exposure damaged the brush border membrane by decreasing the number of intestinal microvilli, which decreased the surface area available for nutrient absorption. SiO2 NP exposure at physiologically relevant doses ultimately caused adverse outcomes in an in vitro model.
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影响因子：
17.1
作者：
Bouwmeester, Hans;Poortman, Jenneke;Hendriksen, Peter J. M.
通讯作者：
Hendriksen, Peter J. M.
影响因子：
4.3
作者：
Contado, Catia;Mejia, Jorge;Lucas, Stephane
通讯作者：
Lucas, Stephane
影响因子：
3.8
作者：
Di Cristo, Luisana;Movia, Dania;Bergamaschi, Enrico
通讯作者：
Bergamaschi, Enrico
影响因子：
4.8
作者：
Becker, C.;Hammerle-Fickinger, A.;Pfaffl, M. W.
通讯作者：
Pfaffl, M. W.
影响因子：
5
作者：
Bhattacharjee, Sourav;Ershov, Dmitry;Marcelis, Antonius T. M.
通讯作者：
Marcelis, Antonius T. M.